Anti-PTGIS antibody produced in rabbit

HPA014193    Human Protein Atlas characterization data

The gene PTGIS (prostaglandin I2 synthase) encodes a protein belonging to the cytochrome P450 superfamily of enzymes. The encoded protein is also referred to as prostacyclin synthase. The gene is made up of 10 exons spanning a length of approximately 60kb. It is mapped to human chromosome 20q13.11–q13.13. The 5′ flanking section of the gene contains the core binding sequence (GAGACC) of the shear stress responsive element. Two different mRNA transcripts have been found in human aorta and lung, one 6kb and the other spanning a length of 3.3kb.

Prostacyclin synthase recombinant protein epitope signature tag (PrEST)

All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project (www.proteinatlas.org)and as a result, are supported by the most extensive characterization in the industry.

The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. To view these protocols and other useful information about Prestige Antibodies and the HPA, visit sigma.com/prestige.

Prostacyclin synthase encoded by the gene PTGIS catalyzes the conversion of PGH2 (Prostaglandin H2) to PGI2 (prostaglandin I2). PGH2 is derived from arachidonic acid by COX1 (cytochrome c oxidase subunit 1) and COX2. PGI2 is found to have antiproliferative effects and chemopreventive properties. Intracellular PGI2 activates endogenous PPARδ (peroxisome proliferator activated receptor δ) receptor and triggers apoptosis. Epigenetic inactivation of PTGIS gene has been found frequently in colorectal carcinogenesis.

Prestige Antibodies^® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies^® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.

Every Prestige Antibody is tested in the following ways:

• IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
• Protein array of 364 human recombinant protein fragments.

Corresponding Antigen APREST71877.

Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide

Prestige Antibodies is a registered trademark of sigma-aldrich Co. LLC

rabbit

100

unconjugated

affinity isolated antibody

primary antibodies

polyclonal

Prestige Antibodies^® Powered by Atlas Antibodies

buffered aqueous glycerol solution

human

antibody small pack of 25 μL

recombinant expression
Learn more about Antibody Enhanced Validation

immunoblotting: 0.04-0.4 μg/mL
immunohistochemistry: 1:200-1:500

LTLYGIEALPRTHESQAQDRVHSADVFHTFRQLDRLLPKLARGSLSVGDKDHMCSVKSRLWKLLSPARLARRAHRSKWLESYLLHLEEMGVSEEMQARALVLQLWATQGNMGPAAFWLLLFLLKNPEALAAVRGELESILWQAEQ

Q16647

wet ice

−20℃

human ... PTGIS(5740)

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WGK 1

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