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Anti-PKM2 (isoform M2) antibody produced in rabbit

产品编号:3386537
规格:~1.5 mg/mL, affinity isolated antibody
包装规格:200 μL
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
3386537200 μL5650
产品别名

Anti-PKM2 (isoform M2) antibody produced in rabbit

Anti-CTHBP

Anti-PK3

Anti-Pyruvate Kinase, MUSCLE (isoform M1)

Anti-OIP3(OPA-interacting protein 3)

Anti-TCB

Anti-THBP1 (thyroid hormone-binding protein, cytosolic)

Anti-PKM

基本信息
NACRES
NA.44
General description【一般描述】
Pyruvate Kinase (PK) has four known isoforms, namely, L, R, M1 and M2. PK isoform M1 is expressed during the development of the embryo and is the dominant isoform in cardiac, brain and skeletal muscle tissues. The M2 isoform has been linked to cancer metastasis .
Specificity【特异性】
Anti-PKM2 (isoform M2) antibody is specific for human PKM2 (isoform M2). The product is expected to react with rat and mouse PKM2. In immunoblotting, detection of the PKM2 (isoform M2) band is specifically inhibited by the PKM2 (isoform M2) immunizing peptide.
Application【应用】
Anti-PKM2 (isoform M2) antibody produced in rabbit has been used in:
  • chromatin immunoprecipitation (chip) assay
  • western blotting
  • immunofluorescence
  • immunohistochemistry

Biochem/physiol Actions【生化/生理作用】
Pyruvate Kinase (PK) is a key regulatory enzyme in glycolysis. Tumor cells have been reported to exclusively overexpress the embryonic M2 isoform. The tumor metabolome is characterized by high glycolytic turnover rate and tumor cells are able to proliferate under conditions of aerobic glycolysis, known as the Warburg effect. Knockdown of the M2 isoform in human cancer cell lines and its replacement by the M1 isoform has been shown to lead to reversal of the Warburg effect, and reduced ability to form tumors in mouse xenografts. Phosphorylation of the M2 isoform at Tyr105 inhibits its activity and is common in human cancers, suggesting that Tyr105 is a critical metabolic switch in cancer cells that promotes tumorigenesis.
Physical form【外形】
Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.
产品性质
biological source【生物来源】
rabbit
conjugate【偶联物】
unconjugated
antibody form【抗体形式】
affinity isolated antibody
antibody product type
primary antibodies
clone【克隆】
polyclonal
form【形式】
buffered aqueous solution
mol wt【分子量】
antigen ~60 kDa
species reactivity
human
concentration【浓度】
~1.5 mg/mL
technique(s)
immunohistochemistry: 10-20 μg/mL using formalin-fixed paraffin embedded human colon carcinoma
immunoprecipitation (IP): 2-4 μg using HT29 cell lysates
indirect immunofluorescence: 4-8 μg/mL using HeLa cells
western blot: 0.5-1.0 μg/mL using HeLa cell extracts
shipped in【运输】
dry ice
storage temp.【储存温度】
−20℃
Gene Information
human ... PKM2(5315)
产品说明
Disclaimer【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
安全信息
Storage Class Code【储存分类代码】
12 - Non Combustible Liquids
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