Anti-phospho-PAK1/2/3 (pThr⁴²³) antibody produced in rabbit

MFCD06411891

NA.44

p21-activated kinases are a family of highly conserved serine/threonine protein kinases that are activated by small GTPases of Cdc42 and Rac families. There are 2 groups of PAKs in mammals, group A and B. The group A members are three isoforms, PAK1, PAK2, and PAK3 (also known as α-PAK, γ-PAK, and β-PAK, respectively). PAKs are actively involved in regulation of MAPK pathways by phosphorylation of Raf-1 and MEK-1. They also regulate cytoskeletal reorganization by interaction with GTPases. Autophosphorylation of threonine 423 (threonine 402 for PAK2 and threonine 421 for PAK3), catalyzed by Cdc42, is required for activation of PAK.
The antibody detects human PAK1, PAK2 and PAK3, phosphorylated at Thr⁴²³. Other species with 100% PAK homology are expected to react.

synthetic phosphopeptide derived from the region of PAK that contains threonine 423.

A recommended working concentration of 0.1-1.0 μg/mL is determined by immunoblotting using Histagged wild-type and S139A/T421A double mutant PAK3.

Solution in Dulbecco′s phosphate buffered saline (without Mg²⁺ and Ca²⁺), pH 7.3, with 50% glycerol, 1.0 mg/mL BSA (IgG and protease free) and 0.05% sodium azide.

rabbit

200

unconjugated

affinity isolated antibody

primary antibodies

polyclonal

buffered aqueous glycerol solution

human

western blot: 1:1,000 using His-tagged wild-type and S139A/T421A double mutant PAK3.

Q13153

wet ice

−20℃

human ... PAK1(5058)

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