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Anti-phospho-Insulin Receptor Substrate-1 (pSer312) antibody produced in rabbit
| 产品编号： | 3384923 |
| 规格： | affinity isolated antibody, buffered aqueous glycerol solution |
| 包装规格： | 1 VIAL |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Anti-phospho-Insulin Receptor Substrate-1 (pSer312) antibody produced in rabbit
Anti-phospho-IRS-1(pSer312)
基本信息
| MDL number | MFCD03096979 |
| NACRES | NA.41 |
| General description【一般描述】 | Insulin receptor substrate-1 (IRS-1) is among the four IRS proteins (IRS-1-IRS-4) that mediate signalling by insulin receptor (IR) and insulin-like growth factor (IGF) receptor. IRS-1 has a pleckstrin homology domain and a phosphotyrosine-binding domain. Phosphorylation of the Tyr1179 residue in the SH-1 negatively regulates insulin. IRS-1 has the abililty to translocate to the nucleus, the mechanism of which is not very clear. Estrogen receptors (ER) α and β have a strong affinity to IRS-1, an important interaction in breast cancers and medulloblastomas. The expression of IRS-1 in prostate cancer is associated with lower tumor cell invasiveness. Along with ER α, IRS-1 also modulates transcriptional activity involved in the development of breast and prostate cancer. The signalling pathway involving IGF-IR-IRS-1 in cells expressing JCV T-antigen has been reported to affect the proliferation, survival and DNA repair in the cells. Phosphorylation of IRS-1 at Ser312 and Ser616 results in the negative regulation of insulin signalling in humans Anti-phospho-IRS-1 (pSer312) specifically recognizes human IRS-1 (pSer312). It may react with mouse and rat (100% homology) IRS-1. |
| Immunogen【免疫原】 | synthetic phosphopeptide derived from the region of IRS-1 that contains serine 312. This sequence is 100% homologous with mouse and rat IRS-1. |
| Application【应用】 | Anti-phospho-IRS-1 (pSer312) antibody may be used for detection by immunoblotting, in stimulated CHO cell line expressing human insulin receptor, at a working dilution of 1:1000. |
| Physical form【外形】 | Solution in Dulbecco′s phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 1.0 mg/mL BSA (IgG, protease free) and 0.05% sodium azide. |
产品性质
| biological source【生物来源】 | rabbit |
| Quality Level【质量水平】 | 200 |
| conjugate【偶联物】 | unconjugated |
| antibody form【抗体形式】 | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone【克隆】 | polyclonal |
| form【形式】 | buffered aqueous glycerol solution |
| species reactivity | human |
| technique(s) | western blot: 1:1000 using stimulated CHO cell line |
| UniProt accession no.【UniProt登记号】 | P35568 |
| shipped in【运输】 | wet ice |
| storage temp.【储存温度】 | −20℃ |
| Gene Information | human ... IRS1(3667) mouse ... Irs1(16367) rat ... Irs1(25467) |
产品说明
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
| Personal Protective Equipment【个人防护装备】 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |
