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Monoclonal Ant-phospho-Cofilin (pSer3) 兔抗
Anti-phospho-Cofilin (pSer3) antibody produced in rabbit
产品编号: | 3384430 |
规格: | IgG fraction of antiserum, buffered aqueous solution |
包装规格: | 200 μL |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
产品别名
Monoclonal Ant-phospho-Cofilin (pSer3) 兔抗
Anti-phospho-Cofilin (pSer3) antibody produced in rabbit
Anti-CFL1
Anti-18 kDa phosphoprotein
Anti-p18
Anti-cofilin 1 (non-muscle)
基本信息
MDL number | MFCD04040757 |
NACRES | NA.41 |
General description【一般描述】 | Cofilin is a small phosphoinositide-sensitive actin-binding protein capable of depolymerizing actin-filaments in vitro. It has gelsolin-like actin filament-severing protein similar to destrin/ADF (Actin Depolymerizing Factor). Cofilin has has a short β-strand at the C terminus. In mammals it has two isoforms: non-muscle (NM-CF, CF-L1) and muscle (M-CF, CF-L2). The protein is ubiquitiously present in tissues of eukaryotes and is especially abundant in neuronal tissues. It can shuttle between the cytoplasm and the nucleus in response to various stresses or signals, and may translocate from the cytoplasm to the plasma membrane in various cells. |
Immunogen【免疫原】 | synthetic phosphopeptide corresponding to amino acids 2-9 (pSer3) of human cofilin with a C-terminal added cysteine, conjugated to KLH. This sequence is identical in mouse, rat, and pig. |
Application【应用】 | Anti-phospho-Cofilin antibody was used:
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Biochem/physiol Actions【生化/生理作用】 | Cofilin binds stoichiometrically to monomeric G-actin and actin protomers in filaments in an apparently pH-dependent, Ca2+ independent manner. Cofilin intercalates between longitudinally associated actin monomers within the filament and distorts its helical twist.It cleaves the filaments and accelerates actin subunits dissociation from their ‘pointed′ ends under specific conditions. Cofilin is essential for viability and is important for many cellular processes involving actin remodeling such as motility at the leading edge of cells, polarized cell growth, endocytosis, phagocytosis, cellular activation, cytokinesis, and pathogen intracellular motility. It′s activity is regulated through reversible phosphorylation and dephosphorylation. In phosphorylated form, it behaves inactive and unable to bond with actin. Phosphorylation of cofilin is regulated in vertebrates by at least four protein kinases: LIM Kinase 1, LIM Kinase 2, Testicular Kinase 1, and Testicular Kinase 2. The dephosphorylation of cofilin enables its actin severing and depolymerizing activity and drives directional cell motility, thus providing a simple phosphoregulatory mechanism for actin reorganization. |
Physical form【外形】 | 0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。 |
产品性质
biological source【生物来源】 | rabbit |
Quality Level【质量水平】 | 200 |
conjugate【偶联物】 | unconjugated |
antibody form【抗体形式】 | IgG fraction of antiserum |
antibody product type | primary antibodies |
clone【克隆】 | polyclonal |
form【形式】 | buffered aqueous solution |
mol wt【分子量】 | antigen ~19 kDa |
species reactivity | mouse, rat (predicted), pig (predicted), human |
technique(s) | western blot: 1:2,000-1:4,000 using whole extracts of HeLa human epithelioid carcinoma and mouse NIH3T3 cells |
UniProt accession no.【UniProt登记号】 | P23528 |
shipped in【运输】 | dry ice |
storage temp.【储存温度】 | −20℃ |
Gene Information | human ... CFL1(1072) mouse ... Cfl1(12631) rat ... Cfl1(29271) |
产品说明
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 3 |
Personal Protective Equipment【个人防护装备】 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |