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Monoclonal Ant-phospho-Cofilin (pSer3) 兔抗

Anti-phospho-Cofilin (pSer3) antibody produced in rabbit
产品编号:3384430
规格:IgG fraction of antiserum, buffered aqueous solution
包装规格:200 μL
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
3384430200 μL6560
产品别名

Monoclonal Ant-phospho-Cofilin (pSer3) 兔抗

Anti-phospho-Cofilin (pSer3) antibody produced in rabbit

Anti-CFL1

Anti-18 kDa phosphoprotein

Anti-p18

Anti-cofilin 1 (non-muscle)

基本信息
MDL number
MFCD04040757
NACRES
NA.41
General description【一般描述】
Cofilin is a small phosphoinositide-sensitive actin-binding protein capable of depolymerizing actin-filaments in vitro. It has gelsolin-like actin filament-severing protein similar to destrin/ADF (Actin Depolymerizing Factor). Cofilin has has a short β-strand at the C terminus. In mammals it has two isoforms: non-muscle (NM-CF, CF-L1) and muscle (M-CF, CF-L2). The protein is ubiquitiously present in tissues of eukaryotes and is especially abundant in neuronal tissues. It can shuttle between the cytoplasm and the nucleus in response to various stresses or signals, and may translocate from the cytoplasm to the plasma membrane in various cells.
Immunogen【免疫原】
synthetic phosphopeptide corresponding to amino acids 2-9 (pSer3) of human cofilin with a C-terminal added cysteine, conjugated to KLH. This sequence is identical in mouse, rat, and pig.
Application【应用】
Anti-phospho-Cofilin antibody was used:
  • as primary antibody for immunoblottingto to study the role of the Rac1 GTPase.
  • for western blot analysis in a comparative study of signals induced by integrin ligation during cell attachment, mechanical force from intracellular contraction, or cell stretching by external force.
  • as primary antibody for western blotting in a study to detect the level of cofilin.
Biochem/physiol Actions【生化/生理作用】
Cofilin binds stoichiometrically to monomeric G-actin and actin protomers in filaments in an apparently pH-dependent, Ca2+ independent manner. Cofilin intercalates between longitudinally associated actin monomers within the filament and distorts its helical twist.It cleaves the filaments and accelerates actin subunits dissociation from their ‘pointed′ ends under specific conditions. Cofilin is essential for viability and is important for many cellular processes involving actin remodeling such as motility at the leading edge of cells, polarized cell growth, endocytosis, phagocytosis, cellular activation, cytokinesis, and pathogen intracellular motility. It′s activity is regulated through reversible phosphorylation and dephosphorylation. In phosphorylated form, it behaves inactive and unable to bond with actin. Phosphorylation of cofilin is regulated in vertebrates by at least four protein kinases: LIM Kinase 1, LIM Kinase 2, Testicular Kinase 1, and Testicular Kinase 2. The dephosphorylation of cofilin enables its actin severing and depolymerizing activity and drives directional cell motility, thus providing a simple phosphoregulatory mechanism for actin reorganization.
Physical form【外形】
0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。
产品性质
biological source【生物来源】
rabbit
Quality Level【质量水平】
200
conjugate【偶联物】
unconjugated
antibody form【抗体形式】
IgG fraction of antiserum
antibody product type
primary antibodies
clone【克隆】
polyclonal
form【形式】
buffered aqueous solution
mol wt【分子量】
antigen ~19 kDa
species reactivity
mouse, rat (predicted), pig (predicted), human
technique(s)
western blot: 1:2,000-1:4,000 using whole extracts of HeLa human epithelioid carcinoma and mouse NIH3T3 cells
UniProt accession no.【UniProt登记号】
P23528
shipped in【运输】
dry ice
storage temp.【储存温度】
−20℃
Gene Information
human ... CFL1(1072)
mouse ... Cfl1(12631)
rat ... Cfl1(29271)
产品说明
Disclaimer【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
安全信息
Storage Class Code【储存分类代码】
12 - Non Combustible Liquids
WGK
WGK 3
Personal Protective Equipment【个人防护装备】
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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