Anti-Gα_q11, C-Terminal antibody produced in rabbit

MFCD00239680

NA.41

G-proteins are membrane associated heterotrimeric proteins comprising of α-, β-, and γ-subunits. The α-subunit contains a guanine-binding domain that is in an inactive state when it is occupied by GDP. Upon activation, GDP is replaced with GTP, causing the dissociation of the α-subunit from the βγ-subunit complex. This enables the Gα-GTP complex to bind to and regulate specific signaling pathways. GTP is then hydrolyzed, allowing for re-association of the α-subunit with the βγ-subunit complex.

The sequence is highly conserved among species. The antibody is specific for Gα_q11 and Gα_q.

synthetic peptide corresponding to amino acids 350-359 of Gα_q11 or amino acids 344-353 of Gα_q.

Anti-Gα_q11, C-terminal antibody produced in rabbit is suitable for immunoprecipitation and western blot analysis at a working dilution of 1:500-1:2000 using 20μg of rat brain microsomal preparation.

G_q α (G₁₁α) regulates the action of phospholipase C (PLC), and results in the release of other intracellular messengers through the inositol phosphate pathway, leading to the release of Ca²⁺ and the activation of phosphorylation.

Solution in phosphate buffered saline, pH 7.4.

rabbit

200

unconjugated

IgG fraction of antiserum

primary antibodies

polyclonal

buffered aqueous solution

human, mouse

immunoprecipitation (IP): suitable
western blot: 1:500-1:2,000 using rat brain microsomal preparation (20 μg)

P50148

wet ice

−20℃

human ... GNAQ(2776)
mouse ... Gnaq(14682)

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