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Anti-dimethyl-Histone H3 (diMe-Lys9) antibody produced in rabbit
| 产品编号： | 3357570 |
| 规格： | IgG fraction of antiserum, buffered aqueous solution |
| 包装规格： | 200 μL |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Anti-dimethyl-Histone H3 (diMe-Lys9) antibody produced in rabbit
Anti-H3K9me2
基本信息
| MDL number | MFCD06798437 |
| NACRES | NA.41 |
| General description【一般描述】 | Histone methylation is a complex, dynamic process involved in a number of processes, including transcriptional regulation, chromatin condensation, mitosis and heterochromatin assembly. Conserved lysine residues in the N-terminal tail domains of histone H3, Lys4, Lys9 and Lys27 are the preferred sites of methylation. SET domain-, site-specific histone methyltransferases (HMTases) are involved in methylation of Lys9 in histone 3. |
| Specificity【特异性】 | ChIP validated |
| Immunogen【免疫原】 | synthetic methylated peptide corresponding to amino acids 5-13 (diMe-Lys9) of human histone H3, conjugated to KLH. This sequence is identical in many species including mouse, rat, bovine, chicken, frog, Drosophila, C. elegans, tetrahymena, and Arabidopsis thaliana histone H3. |
| Application【应用】 | Anti-dimethyl-Histone H3 (diMe-Lys9) antibody produced in rabbit has been used in immunoblotting and chromatin immunoprecipitation assay. |
| Biochem/physiol Actions【生化/生理作用】 | Mono- and dimethylation of H3 at Lys9 are intrinsically linked to epigenetic silencing and heterochromatin assembly. Methylation of H3 at Lys9 generates a binding site for HP1 proteins, a family of heterochromatic adaptor proteins implicated in both gene silencing and in the organization of higher order chromatin. |
| Physical form【外形】 | 0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。 |
产品性质
| biological source【生物来源】 | rabbit |
| Quality Level【质量水平】 | 200 |
| conjugate【偶联物】 | unconjugated |
| antibody form【抗体形式】 | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| clone【克隆】 | polyclonal |
| form【形式】 | buffered aqueous solution |
| mol wt【分子量】 | antigen 17 kDa |
| species reactivity | bovine, human, Drosophila, Arabidopsis thaliana, rat, frog, chicken, Caenorhabditis elegans, mouse |
| technique(s) | ChIP: suitable microarray: suitable western blot: 1:1,000-1:2,000 using whole extract of human epitheloid carcinoma HeLa cell line |
| UniProt accession no.【UniProt登记号】 | P68431 |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −20℃ |
| Gene Information | human ... H3F3A(3020), H3F3B(3021), HIST1H3A(8350), HIST1H3B(8358), HIST1H3C(8352), HIST1H3D(8351), HIST1H3E(8353), HIST1H3F(8968), HIST1H3G(8355), HIST1H3H(8357), HIST1H3I(8354), HIST1H3J(8356), HIST2H3A(333932), HIST2H3C(126961), HIST3H3(8290) mouse ... H3c10(319152), H3c11(319153), H3f3a(15078), H3f3b(15081), Hist1h3a(360198), Hist1h3b(319150), Hist1h3c(319148), Hist1h3d(319149), Hist1h3e(319151), Hist1h3f(260423), Hist1h3g(97908), Hist2h3b(319154), Hist2h3c1(15077), Hist2h3c2(97114) rat ... H3f3b(117056) |
产品说明
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | nwg |
