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Anti-DFF45, N-Terminal antibody produced in rabbit
| 产品编号： | 3357290 |
| 规格： | IgG fraction of antiserum, buffered aqueous solution |
| 包装规格： | 0.1 MG |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Anti-DFF45, N-Terminal antibody produced in rabbit
Anti-ICAD
基本信息
| MDL number | MFCD02096210 |
| NACRES | NA.41 |
| General description【一般描述】 | DFF exists as an inactive cytoplasmic protein until activated by apoptotic signals. DFF40, the nuclease responsible for DNA fragmentation during apoptosis, exists as a heterodimeric complex with DFF45. |
| Immunogen【免疫原】 | synthetic peptide corresponding to amino acids 2-21 of the N-terminal region of human DFF45/ICAD (DNA Fragmentation Factor 45/Inhibitor of Caspase-3 Activated DNase I). |
| Application【应用】 | Anti-DFF45 antibody produced in rabbit is suitable for immunoblotting at a working concentration of 0.5μg/mL using a total HeLa cell lysate. |
| Biochem/physiol Actions【生化/生理作用】 | DFF45 functions as both a chaperone, mediating the correct folding of DFF40, and an inhibitor of DFF40. DFF45 is cleaved by caspase-3 at two sites in response to apoptotic signals. This releases DFF40, the active nuclease. DFF40 seems to oligomerize, forming a large, functional complex which breaks down DNA by introducing double strand breaks. Also, DFF40 appears to interact directly with histone H1 that may stimulate its activity. |
| Physical form【外形】 | Solution in phosphate buffered saline containing 0.02% sodium azide. |
产品性质
| biological source【生物来源】 | rabbit |
| Quality Level【质量水平】 | 100 |
| conjugate【偶联物】 | unconjugated |
| antibody form【抗体形式】 | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| clone【克隆】 | polyclonal |
| form【形式】 | buffered aqueous solution |
| mol wt【分子量】 | antigen 45 kDa (non-apoptotic cells) |
| species reactivity | human |
| technique(s) | western blot: 0.5 μg/mL using human HeLa cell extract |
| UniProt accession no.【UniProt登记号】 | O00273 |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −20℃ |
| Gene Information | human ... DFFA(1676) |
