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Anti-DCP2 (C-terminal) antibody produced in rabbit
产品编号: | 3356784 |
规格: | ~1 mg/mL, affinity isolated antibody, buffered aqueous solution |
包装规格: | 200 μL |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
产品别名
Anti-DCP2 (C-terminal) antibody produced in rabbit
Anti-DCP2 decapping enzyme homolog (S. cerevisiae)
Anti-Nudix motif 20
Anti-NUDT20
Anti-Nucleleoside diphosphate-linked moiety X motif 20
基本信息
NACRES | NA.41 |
General description【一般描述】 | Dcp2 colocalizes with Dcp1 in distinct cytoplasmic foci along with other proteins involved in the 5′ to 3′ mRNA decay. These foci are termed PB (processing bodies) or DCP-bodies. hDCP2 contains a highly conserved Nudix (nucleoside diphosphate linked to an X moiety) motif critical for the decapping activity. |
Immunogen【免疫原】 | synthetic peptide corresponding to amino acids 406-420 of human DCP2, conjugated to KLH. The corresponding sequence is identical in rat and mouse. |
Application【应用】 | Anti-DCP2 (C-terminal) antibody has been used in immunocytochemistry and image correlation analysis. It may also be used in immunoblotting. Anti-DCP2 antibody produced in rabbit is suitable for indirect immunofluorescence at a working concentration of 2-5μg/mL using paraformaldehyde-fixed NIH-3T3 cells over-expressing human DCP2 and western blot analysis at a working concentration of 2-4μg/mL using lysates of K-562 and Rat1 cells. Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about us and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if. |
Biochem/physiol Actions【生化/生理作用】 | Dcp2 is an RNA binding protein and can cleave only a cap structure that is linked to an RNA moiety, suggesting that Dcp2 can differentially associate with specific mRNAs. Dcp2 cleaves the m7G mRNA cap in the 5′ to 3′ mRNA decay pathway, in association with Dcp1 and Hedls complex. Decapping is a critical and highly regulated step in the turnover of mRNA which involves decapping enzymes that hydrolyze the cap structure at the 5′ mRNA. Dcp2 is the catalytic subunit, and the mRNA is degraded by the major cytoplasmic 5′ to 3′ exonuclease XRN1. The enzymatic activity of DCP2 is critically dependent on the DCP1 subunit in vivo. |
Physical form【外形】 | 0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。 |
产品性质
biological source【生物来源】 | rabbit |
Quality Level【质量水平】 | 200 |
conjugate【偶联物】 | unconjugated |
antibody form【抗体形式】 | affinity isolated antibody |
antibody product type | primary antibodies |
clone【克隆】 | polyclonal |
form【形式】 | buffered aqueous solution |
mol wt【分子量】 | antigen ~50 kDa |
species reactivity | rat, mouse, human |
concentration【浓度】 | ~1 mg/mL |
technique(s) | indirect immunofluorescence: 2-5 μg/mL using paraformaldehyde-fixed NIH-3T3 celle indirect immunofluorescence: suitable western blot: 2-4 μg/mL using lysaes of K-562 and Rat1 cells |
UniProt accession no.【UniProt登记号】 | Q8IU60 |
shipped in【运输】 | dry ice |
storage temp.【储存温度】 | −20℃ |
Gene Information | human ... DCP2(167227) mouse ... Dcp2(20640) rat ... Dcp2(291604) |
产品说明
Target description【目标描述】 | DCP2 (C-terminal) is a key component of an mRNA-decapping complex required for removal of the 5-prime cap from mRNA prior to its degradation from the 5-prime end. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
Personal Protective Equipment【个人防护装备】 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |