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Anti-CENP-E antibody produced in rabbit
| 产品编号： | 3352886 |
| 规格： | IgG fraction of antiserum, buffered aqueous solution |
| 包装规格： | 200 μL |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Anti-CENP-E antibody produced in rabbit
Anti-Centromere-associated protein-E
基本信息
| MDL number | MFCD05665206 |
| General description【一般描述】 | Centromere-associated protein-E (CENP-E, 312 kDa) is a member of the kinesin superfamily of microtubule motor proteins and is an integral part of kinetochore corona fibers that link centromere to the spindle microtubules. CENP-E localizes to the kinetochore throughout all phases of mitotic chromosome movement from early prometaphase through anaphase A. |
| Immunogen【免疫原】 | synthetic peptide corresponding to amino acids 2545-2563 located near the C-terminus of human CENP-E conjugated to KLH. |
| Application【应用】 | Anti-CENP-E antibody produced in rabbit has been used in:
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| Biochem/physiol Actions【生化/生理作用】 | Centromere-associated protein-E (CENP-E) plays an important role in attachment of kinetochores to spindle microtubules in the alignment of chromosomes and is an essential component of mitotic checkpoint signaling cascade. It functions as a motor in the initial chromosome movement at the mitotic midzone. CENP-E is associated with minus end-directed microtubule motor activity suggesting that CENP-E might be responsible for poleward kinetochore movements in prometaphase and anaphase A. Suppression of CENP-E synthesis by antisense CENP-E yields chromosomes that are chronically mono-oriented with flattened bipolar spindles and generates spindle poles fragments. Depletion of CENP-E leads to profound checkpoint activation and long-term mitotic arrest. CENP-E has been implicated as a binding partner for the mitotic checkpoint kinase BubR1 during mitosis. |
| Physical form【外形】 | 0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。 |
产品性质
| biological source【生物来源】 | rabbit |
| Quality Level【质量水平】 | 200 |
| conjugate【偶联物】 | unconjugated |
| antibody form【抗体形式】 | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| clone【克隆】 | polyclonal |
| form【形式】 | buffered aqueous solution |
| mol wt【分子量】 | antigen ~310 kDa |
| species reactivity | human |
| technique(s) | immunocytochemistry: suitable using human epitheloid carcinoma HeLa cell line. microarray: suitable western blot: 1:2,000 using whole extract of HeLa nuclear cell line |
| UniProt accession no.【UniProt登记号】 | Q02224 |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −20℃ |
| Gene Information | human ... CENPE(1062) |
| packaging【包装】 | 200 μL in glass insert |
产品说明
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | nwg |
