您的位置:首页 > 产品中心 > Anti-CDK4 antibody produced in rabbit
Anti-CDK4 antibody produced in rabbit
产品别名
Anti-CDK4 antibody produced in rabbit
Anti-PSK-J3
Anti-CMM3
基本信息
NACRES | NA.41 |
General description【一般描述】 | The antibody detects endogenous levels of total CDK4 protein. |
Immunogen【免疫原】 | Peptide sequence around aa. 295-299 (L-H-K-D-E), according to the protein NP_000066.1 |
Application【应用】 | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Western Blotting (1 paper) |
Features and Benefits【特点和优势】 | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
Physical form【外形】 | Solution in phosphate-buffered saline containing 0.02% sodium azide and 50% glycerol |
产品性质
biological source【生物来源】 | rabbit |
conjugate【偶联物】 | unconjugated |
antibody form【抗体形式】 | affinity isolated antibody |
antibody product type | primary antibodies |
clone【克隆】 | polyclonal |
form【形式】 | buffered aqueous solution |
mol wt【分子量】 | ~34 kDa |
species reactivity | human |
concentration【浓度】 | 1 mg/mL |
technique(s) | western blot: 1:500-1:1000 |
isotype【同位素/亚型】 | IgG |
immunogen sequence【免疫原序列】 | (L-H-K-D-E) |
NCBI accession no.【NCBI登记号】 | NM_000075.2 |
UniProt accession no.【UniProt登记号】 | P11802 |
shipped in【运输】 | wet ice |
storage temp.【储存温度】 | −20℃ |
Gene Information | human ... CDK4(1019) |
产品说明
Target description【目标描述】 | Cyclin-dependent kinase activity is regulated by T-loop phosphorylation (Thr172 in the case of CDK4), by the abundance of their cyclin partners, and by association with CDK inhibitors of the Cip/Kip or INK family of proteins . The inactive ternary complex of CDK4/cyclin D and p27 Kip1/Cip1 requires extracellular mitogenic stimuli for the release and degradation of p27, which affects progression through the restriction point and pRb-dependent entry into S-phase . The active complex of CDK4/cyclin D targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1/S-phase gene expression . In HeLa cells, upon UV irradiation, upregulation of p16 INK4A association with CDK4/cyclin D3 leads to a G2 delay, implicating CDK4/cyclin D3 activity in progression through the G2-phase of the cell cycle . |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 1 |