Anti-CASP8 antibody produced in rabbit

MFCD01633094

HPA005688    Human Protein Atlas characterization data

Caspase 8 (CASP8) is a cysteine protease, belonging to the cysteine-aspartic acid protease (caspase) family.

Caspase-8 precursor recombinant protein epitope signature tag (PrEST)

Sequence
LGEGKLDILKRVCAQINKSLLKIINDYEEFSKGEELCGVMTISDSPREQDSESQTLDKVYQMKSKPRGYCLIINNHNFAKAREKVPKLHSIRDRNGTHLDAGALTTTFEELHFEIKPHDDCTVEQIYEILKIYQLMDHSNMDCFICC

All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project (www.proteinatlas.org)and as a result, are supported by the most extensive characterization in the industry.

The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. To view these protocols and other useful information about Prestige Antibodies and the HPA, visit sigma.com/prestige.

Caspase 8 (CASP8) is the first protease of the activation cascade of caspases responsible for the tumor necrosis factor receptor superfamily member 6 (TNFRSF6/FAS) mediated and tumor necrosis factor receptor superfamily member F1A (TNFRSF1A) induced cell death. After binding to the adapter molecule, Fas-associated via death domain (FADD) recruits it to a receptor. This results in the formation of an aggregation called death-inducing signaling complex (DISC) which carries out the proteolytic activation of CASP8. Then its active dimeric form is liberated from the DISC and activates downstream apoptotic proteases. It then cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. CASP8 also participates in Granzyme (GZMB) apoptotic pathways. It hydrolyzes a small-molecule substrate, Ac-Asp-Glu-Val-Asp-AMC.CASP8 is a modulator of p85a Rab-GAP activity and endosomal trafficking. The interaction between it and p85a causes Rab5 GTP loading, changes endosomal trafficking and at the edge of the cell, Rab5-positive endosomes are seen.

Prestige Antibodies^® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies^® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.

Every Prestige Antibody is tested in the following ways:

• IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
• Protein array of 364 human recombinant protein fragments.

Corresponding Antigen APREST78230.

Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide

Prestige Antibodies is a registered trademark of sigma-aldrich Co. LLC

rabbit

100

unconjugated

affinity isolated antibody

primary antibodies

polyclonal

Prestige Antibodies^® Powered by Atlas Antibodies

buffered aqueous glycerol solution

human

antibody small pack of 25 μL

immunoblotting: 0.04-0.4 μg/mL
immunohistochemistry: 1:50-1:200

Q14790

wet ice

−20℃

human ... CASP8(841)

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

10 - Combustible liquids

WGK 1

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)