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X1/5

产品编号:3260323
规格:95051229
包装规格:5μg,1 VIAL
产品类别:进口试剂
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
32603235μg4630
32603231 VIAL11270
产品性质
biological source【生物来源】
human cervix
growth mode【生长模式】
Adherent
karyotype【核型】
Not specified
morphology【形态学】
Epithelial
products【产品】
Not specified
receptors【受体】
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)【相关疾病】
cancer
shipped in【运输】
dry ice
storage temp.【储存温度】
−196℃
基本信息
Cell Line Origin【细胞系来源】
Human (HeLa) with tTA
Cell Line Description【细胞系描述】
X1/5 is a HeLa cell line stably transfected with a tet repressor fused with the activating domain of virion protein 16 of herpes simplex virus thus generating a tetracycline-controlled transactivator (tTA) This stimulates transcription from a minimal promoter sequence derived from the human cytomegalovirus promoter IE combined with tet operator sequences. Integration of a luciferase gene controlled by a tTA-dependent promoter into this tTA producing HeLa cell line leads to high levels of tetracycline-sensitive luciferase expression. The luciferase activity can be regulated over up to five orders of magnitude depending on the concentration of tetracycline in the culture medium (0-1ug/ml). Specific and stringent control of the activity of an individual gene (luciferase) in the mammalian cell can be monitored. It is also suitable for creation of on/off situations and analysis of mRNA decay rates under physiological conditions
Application【应用】
Specific and stringent control of the activity of an individual gene (luciferase) in a mammalian cell can be monitored. It is also suitable for creation of
Culture Medium【培养基】
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1% Vitamins + 500μg/ml G418 + 300μg/ml Hygromycin + 10% Foetal Bovine Serum (FBS). Freeze cells in 40% medium, 10% Dimethyl Sulphoxide (DMSO) and 50% Foetal Bovine Serum (FBS).
Subculture Routine【传代培养常规】
Split sub-confluent cultures (70-80%) 1:5 to 1:20 i.e. seeding at 5x1,000 to 2x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA. Alternatively cells can be washed with PBS and subcultured after addition of PBS with 0.8mM EDTA (30% of original volume)
Other Notes【其他说明】
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
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