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产品性质
biological source【生物来源】 | mouse blood |
growth mode【生长模式】 | Suspension |
karyotype【核型】 | Not specified |
morphology【形态学】 | Lymphoblast |
products【产品】 | Surface Immunoglobulin M (IgM) |
receptors【受体】 | Not specified |
technique(s) | cell culture | mammalian: suitable |
relevant disease(s)【相关疾病】 | cancer |
shipped in【运输】 | dry ice |
storage temp.【储存温度】 | −196℃ |
基本信息
Cell Line Origin【细胞系来源】 | Mouse B cell lymphoma |
Cell Line Description【细胞系描述】 | Expresses surface IgM but does not secrete Ig. Secretion of IgM can be induced with LPS. WEHI 231 is a B cell lymphoma of BALB/c x NXB F1 origin induced by mineral oil injection. |
Application【应用】 | Tumourigenicity studies |
Culture Medium【培养基】 | DMEM + 2mM Glutamine + 0.05mM 2-Mercaptoethanol (2ME) + 10% Foetal Bovine Serum (FBS). |
Subculture Routine【传代培养常规】 | Split sub-confluent cultures (70-80%) 1:8 to 1:10 i.e. seeding at 2-9x100,000 cells/ml; 5% CO2; 37℃. |
Other Notes【其他说明】 | Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information. |