ULK2 KO MEF (SV40)

mouse embryo

Mouse embryonic fibroblast, autophagy, Unc51-like kinase, SV40-immortalised

Adherent

Fibroblast

cell culture | mammalian: suitable

dry ice

−196℃

Mouse embryonic fibroblasts (MEFs) derived from the ULK1(+/-) ULK2(+/-) knockout mice. MEFs were isolated and cultured and immortalised with SV40 large T-antigen. This cell line can be used to study ULK1 dependent processes in autophagy.

DMEM + 10% FCS +2mM Glutamine

Trypsinize with 0.05% Trypsin/EDTA and inoculate new cultures at 2 x 10⁴ cells per cm². Cultures must be incubated in a humidified 5% CO₂/95% air incubator at 37^oC.

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Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.