ULK1/2 WT MEF (SIM)

mouse embryo

Mouse embryonic fibroblast, autophagy, UnC51-like kinase, spontaneously immortalised

Adherent

Fibroblast

cell culture | mammalian: suitable

dry ice

−196℃

Mouse embryonic fibroblasts (MEFs) derived from ULK1(+/-) ULK2(+/-) knockout mice. MEFs that were isolated and cultured were immortalised by a standard serial passaging protocol. This cell line can be used as a wild-type control with ULK1 knockout mouse embryonic fibroblasts to study ULK1-dependent processes.

DMEM + 10% FCS + 2mM Glutamine

Trypsinize with 0.05% Trypsin/EDTA and inoculate new cultures at 2 x 10⁴ per cm². Cultures must be incubated in a humidified 5% CO₂/95% air incubator at 37^oC.

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.