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内切核酸酶 来源于粘质沙雷氏菌
Turbonuclease from Serratia marcescens
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产品编号 | 包装单位 | 单价(元) | 国内现货 | 国外库存 | 询价单 |
3257461 | 50000 UNITS | 4050 |
产品别名
9025-65-4
内切核酸酶 来源于粘质沙雷氏菌
Turbonuclease from Serratia marcescens
Endonuclease from Serratia marcescens
基本信息
Enzyme Commission (EC) Number【酶学委员会(EC)编号】 | 3.1.30.2 ( BRENDA | IUBMB ) |
MDL number | MFCD00131010 |
General description【一般描述】 | Endonuclease from Serratia marcescens is a dimer containing two identical monomeric units with distinct protein folds. The core contains a six-stranded antiparallel β-sheet flanked by α-helices on either side. Each monomer bears one active site. This enzyme is a magnesium-dependent nucleases. |
Application【应用】 | Turbonuclease from Serratia marcescens has been used for cell lysis during proximity biotinylation assay (BioID) and affinity-purification. It has also been used as a component of lysis buffer for protein extraction from cell lines for affinity purification studies. Turbonuclease has been used in a study to assess the TY3 gag3 spacer effect on intracellular condensation and uncoating. 用于去除蛋白样品中的核酸。 |
Biochem/physiol Actions【生化/生理作用】 | Turbonuclease provides a nuclease treatment by reducing viscosity and degrading RNA, genomic DNA, baculovirus DNA, and unencapsidated vector DNA. Endonuclease from Serratia marcescens is effective against both single- and double-stranded DNA and RNA. It mediates the digestion of the 3′ O—P bond resulting in oligonucleotides ending with 5′ monophosphate. The activity of this enzyme is known to be less affected by the reducing and chaotropic agents. It is highly stable at room temperature. This endonuclease eliminates the undesired nucleic acids in downstream processing. 消化天然或热变性的DNA和RNA。 |
Unit Definition【单位定义】 | One unit will digest sonicated salmon sperm DNA to acid-soluble oligonucleotides equivalent to a ΔA260 of 1.0 in 30 min at pH 8.0 at 37 ℃. |
Physical form【外形】 | Supplied as a solution in 50 mM Tris-HCl, pH 8.0 and 50 mM NaCl |
产品性质
Quality Level【质量水平】 | 200 |
recombinant【重组】 | expressed in E. coli |
form【形式】 | liquid |
concentration【浓度】 | ≥200,000 units/mL |
storage temp.【储存温度】 | −20℃ |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 2 |