Trypsin, TPCK-Treated

232-650-8

NA.56

胰蛋白酶是一种丝氨酸蛋白酶，可特异性水解赖氨酸及精氨酸残基的羧基侧的多肽键。该款修饰后的胰蛋白酶经过甲苯磺酰基-L-氨基联苯氯甲基酮 (TPCK) 处理，可使外源性糜蛋白酶失活。每个包装含8个小瓶，每瓶含25 μg。

Trypsin, TPCK-Treated has been used:

• as a supplement in Dulbecco′s Modified Eagle Medium (DMEM) for porcine delta coronavirus (PDCoV) infection experiments using epithelial-like pig kidney cell line (LLC-PK1)
• to detach the human umbilical vein endothelial cells (HUVEC) for annexin-V/propidium Iodide (PI) staining assay
• in minimum essential medium (MEM) for multicycle replication kinetics

N-p-Tosyl-L-phenylalanine chloromethyl ketone (TPCK) serves as an irreversible inhibitor of chymotrypsin. Trypsin induces human fibrocyte differentiation. Trypsin is widely used in proteomics for protein sample digestion. In cell culture, trypsinization is carried out to dislodge adherent cells from each other and the walls of the culture vessel.

dry ice

−20℃

GHS07,GHS08

Danger

H315 - H319 - H334 - H335

P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Respiratory system

10 - Combustible liquids