胰蛋白酶 来源于猪胰腺

3.4.21.4 ( BRENDA | IUBMB )

MFCD00082094

NA.54

Trypsin consists of a single chain polypeptide of 223 amino acid residues, produced by the removal of the N-terminal hexapeptide from trypsinogen which is cleaved at the Lys - lle peptide bond. The sequence of amino acids is cross-linked by 6 disulfide bridges. This is the native form of trypsin, beta-trypsin. BETA-trypsin can be autolyzed, cleaving at the Lys - Ser residue, to produce alpha-trypsin. Trypsin is a member of the serine protease family.

对于胰蛋白酶消化肽，胰蛋白酶:肽的使用比例约为1:100至1:20。本品的典型用途是使贴壁细胞从培养表面脱落。 使细胞解离所需的胰蛋白酶浓度主要取决于细胞类型和培养物代数。 Trypsins也被用于细胞培养过程中的细胞再悬浮，在蛋白质组学研究中用于蛋白质的消化，以及在各种凝胶消化中。 其他的应用包括通过基于膜的技术评估结晶，以及在一项研究中确定蛋白质折叠速率和产量可以被动力学陷阱的存在所限制。
For use in immunohistochemical procedures to enhance staining and to unmask antigens after routine fixation and processing.

胰蛋白酶可切割赖氨酸和精氨酸残基的C末端肽段。 如果酸性残基位于切割位点的任意一侧，则该反应的水解速度减慢，并且如果脯氨酸残基位于切割位点的羧基侧，则水解停止。 胰蛋白酶活性的最佳pH条件为7-9。 胰蛋白酶还可以切割氨基酸合成衍生物的酯和酰胺键。 将EDTA添加到胰蛋白酶溶液中作为螯合剂，用于中和钙离子和镁离子以防止其抑制胰蛋白酶活性。 去除这些离子会增加酶活性。

丝氨酸蛋白酶抑制剂(包括DFP、TLCK、APMSF、AEBSEF和抑肽酶等)会抑制胰蛋白酶。

Solutions in 1 mM HCl are stable for 1 year in aliquots and stored at -20℃. The presence of Ca²⁺ will also diminish the self-autolysis of trypsin and maintain its stability in solution. Trypsin will also retain most of its activity in 2.0 M urea, 2.0 M guanidine HCl, or 0.1% (w/v) SDS.

一个BAEE单位以BAEE作为底物，在25℃、pH 7.6条件下每分钟每毫升产生0.001的ΔA₂₅₃ 。一个BTEE单位=320个ATEE单位。反应体积=3.2mL(1cm的光径)。

This product is a lyophilized powder soluble in Hank′s Balanced Salt Solution at 25 mg/mL.
For applications that require EDTA, solubilizing trypsin should be done with a buffered salt solution contaiing no Ca²⁺ or Mg²⁺.

200

tablet

90-110% (compared to standard)

23.8 kDa

−20℃

GHS08

Danger

H334

P261 - P284 - P304 + P340 + P312 - P501

Resp. Sens. 1

11 - Combustible Solids

WGK 1

dust mask type N95 (US), Eyeshields, Faceshields, Gloves