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®缓冲剂
Trizma® Pre-Set crystals
| 产品编号： | 3256282 |
| 规格： | BioPerformance Certified, pH 8.5, average Mw 131.4 |
| 包装规格： | 100 G，250 G |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
®缓冲剂
Trizma® Pre-Set crystals
Tris crystals
Tris 晶体
基本信息
| NACRES | NA.25 |
| Application【应用】 | Dissolve in water to make a 0.1M solution. No pH adjustment is necessary. Guaranteed accuracy: ±0.1 pH units. Tris has a significant temperature coefficient, so select the mixture to give the desired final pH at the desired temperature. From 5 ℃ to 25 ℃, the pH decreases an average of 0.03 pH units per ℃. From 25 ℃ to 37 ℃, the pH decreases an average of 0.025 pH units per ℃. If a different concentration is desired, note that there is a concentration effect on the pH of the solution. 该产品经细胞培养测试。它还经过了低内毒素水平检测并分析了是否已去除核酸酶和蛋白酶。 |
| Other Notes【其他说明】 | This product is a pre-mixed combination of Trizma base and Trizma HCl that can be used to prepare a common pH value for Tris buffer. 某些pH电极不能使用Tris缓冲液提供准确的结果。 |
| Preparation Note【制备说明】 | Trizma溶液可高压灭菌。 |
| Legal Information【法律信息】 | Trizma is a registered trademark of sigma-aldrich Co. LLC |
产品性质
| Quality Level【质量水平】 | 200 |
| grade【等级】 | BioPerformance Certified |
| form【形式】 | crystalline powder |
| mol wt【分子量】 | average Mw 131.4 |
| technique(s) | cell culture | mammalian: suitable |
| impurities【杂质】 | DNase, RNase, Protease, NICKase, none detected endotoxin, tested total aerobic microbial count, tested ≤0.05 A290 ≤0.5% water (Karl Fischer) ≤5 ppm Heavy metals (as Pb) |
| pH【pH值(酸碱度)】 | 8.22 (37 ℃, 0.1 M)(lit.) 8.50 (25 ℃, 0.1 M)(lit.) 8.5 9.09 (5 ℃, 0.1 M)(lit.) |
| useful pH range【有效pH范围】 | 7.0 - 9.0 |
| solubility【溶解性】 | water: 0.66 g/mL, clear, colorless |
| application(s) | diagnostic assay manufacturing |
| packaging【包装】 | 100, 250 g in poly bottle |
安全信息
| Storage Class Code【储存分类代码】 | 11 - Combustible Solids |
| WGK | WGK 1 |
| Personal Protective Equipment【个人防护装备】 | dust mask type N95 (US), Eyeshields, Gloves |
