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产品性质
| biological source【生物来源】 | mouse testis |
| growth mode【生长模式】 | Adherent |
| karyotype【核型】 | Not specified |
| morphology【形态学】 | Not specified |
| products【产品】 | Prostaglandin F2a |
| receptors【受体】 | Epidermal growth factor (EGF), Androgen, Oestrogen, Progesterone |
| technique(s) | cell culture | mammalian: suitable |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −196℃ |
基本信息
| Cell Line Origin【细胞系来源】 | Mouse BALB/c testis, Leydig cell |
| Cell Line Description【细胞系描述】 | Originated from primary cultures of Leydig cell enriched preparations from normal testes of 11-13 day old BALB/c nu/+ mice. Characteristics similar to primary cultures of Leydig cells. Responds to leuteinising hormone but not FSH. In the presence of LH the cells are capable of metabolising cholesterol. These cells are non-tumourigenic. |
| Application【应用】 | Hormone studies |
| Culture Medium【培养基】 | Ham′s F12 + DMEM (1:1) + 2mM Glutamine + 5% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS). |
| Subculture Routine【传代培养常规】 | Split sub-confluent cultures (70-80%) 1:5 to 1:200 i.e. seeding at 1x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37²C. |
| Other Notes【其他说明】 | Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information. |
