Sulfatase from Aerobacter aerogenes

3.1.6.1 ( BRENDA | IUBMB )

MFCD00132399

NA.54

Sulfatases comprise Cys/Ser-X-Pro-X-Arg motif that is conserved in their active sites.

Sulfatase from Aerobacter aerogenes has been used:

• as a deconjugation enzyme for treating plasma samples for quercetin quantification using liquid chromatography with tandem mass spectrometry (LC/MS/MS) analyses
• in fluorescence intensity-based enzymatic assay with an activity-based probe probe 1
• to treat sulfatide liposomes to remove the 3-O-sulfogalactosyde head from sulfatides

Sulfatases hydrolyze sulfate ester bonds to generate inorganic sulfates. Microbial sulfatases participate in sulfur scavenging and are essential enzymes for the utilization of sulfur. They may be associated with pathogenesis. Commercially available sulfatase from Aerobacter aerogenes is useful as a deconjugation enzyme for the removal of glucuronate and sulfate moieties from conjugates. Sulfatases find application in industry and agriculture.

One unit will hydrolyze 1.0 μmole of p-nitrophenyl sulfate per min at pH 7.1 at 37 ℃.

Solution in 50% glycerol containing 0.01 M Tris, pH 7.5.

200

Type VI

buffered aqueous glycerol solution

2-5 units/mg protein (biuret)

~41 kDa

10-20 units/mL

β-Glucuronidase ≤10 U/mL

wet ice

−20℃

10 - Combustible liquids

WGK 2