鞘磷酯酶 来源于蜡样芽胞杆菌

3.1.4.12 ( BRENDA | IUBMB )

MFCD00132362

NA.54

Sphingomyelinase (SMase) is a hydrolase, of 34.2 kDa and its active site contain as a minimum of two magnesium atoms.

Sphingomyelinase has been used in a study to assess the interaction of actin with the HIV-1 accessory protein Nef. Sphingomyelinase has also been used in a study to investigate X-ray scattering as a quality-control tool for liposomal drug-delivery systems.
Sphingomyelinase from Bacillus cereus has been used:

• in the study to analyse the effect of sphingomyelinase on Rubella virus infectivity in cell lines
• in lipoprotein modification for the generation of cholesterol crystals in vitro
• to aggregate LDL (low-density lipoprotein)

启动形成基于鞘磷脂的第二信使。激活 MAPK(丝裂原活化蛋白激酶)和 SAPKs(压力激活蛋白激酶)；从鞘磷脂生成神经酰胺。
Sphingomyelin phosphodiesterase (SMPD) participates in the metabolic reactions comprising sphingomyelin. SMPD has the ability to prevent colorectal carcinoma and colitis.

One unit will hydrolyze 1.0 μmole of TNPAL-sphingomyelin per min at pH 7.4 at 37 ℃.

Solution in 50% glycerol containing 50 mM Tris-HCl, pH 7.5.

buffered aqueous glycerol solution

200

≥100 units/mg protein (Lowry)

34.2 kDa

Phospholipase C ≤1%

2-8℃

Bacillus cereus ATCC 10987 ... spH(2751530)

12 - Non Combustible Liquids

WGK 3