您的位置:首页 > 产品中心 > Sirtuin 2(SIRT2)抑制剂筛选测定试剂盒
Sirtuin 2(SIRT2)抑制剂筛选测定试剂盒
Sirtuin 2 (SIRT2) Inhibitor Screening Assay Kit
产品别名
Sirtuin 2(SIRT2)抑制剂筛选测定试剂盒
Sirtuin 2 (SIRT2) Inhibitor Screening Assay Kit
基本信息
NACRES | NA.41 |
General description【一般描述】 | Sirtuin 2 (SIRT2) is a cytoplasmic protein and is part of the sirtuin family of proteins. Sirtuin family contains a sirtuin core domain and isgrouped into four classes with SIRT2 being a member of class I. SIRT2 colocalizes with microtubules. The gene encoding this protein is localized on human chromosome 19q13.2. With Sigma′s Sirtuin Inhibitor Screening Kit, Sirtuin deacetylates the Substrate, and then a Developer cleaves the deacetylated substrate to release a fluorescent group, the latter of which can be detected at Ex/Em = 395/541 nm. In the presence of a SIRT inhibitor, the deacetylation will be impeded, preventing substrate cleavage and release of the fluorescent group. The kit provides a rapid, simple, sensitive, and reliable test, which is suitable for both low and high-throughput screening of SIRT2 inhibitors. A positive control inhibitor, Nicotinamide, is included to compare with the efficacy of the test inhibitors. |
Application【应用】 | Sirtuin 2 (SIRT2) inhibitor screening assay kit has been used for acetyl peptide deacetylase assay. |
Biochem/physiol Actions【生化/生理作用】 | Sirtuin 2 (SIRT2) maintains the integrity of the genome. Inhibition of SIRT2 can lead to neuroprotection in cellular and invertebrate models of Huntington′s disease. Huntington′s disease is characterized by increased sterol synthesis in neuronal cells and this process is reversed by SIRT2 inhibition. SIRT2 can deacetylate lys40 of α-tubulin both in vitro and in vivo. Knockdown of SIRT2 by small interfering RNA (siRNA) leads to tubulin hyperacetylation. |
Features and Benefits【特点和优势】 |
|
产品性质
usage【用途】 | 100 assays in 96 well plates |
storage condition【储存条件】 | protect from light |
NCBI accession no.【NCBI登记号】 | NM_001122765 (Mouse) NM_001193286 (Human) |
shipped in【运输】 | wet ice |
storage temp.【储存温度】 | −20℃ |
Gene Information | human ... SIRT2(22933) mouse ... SIRT2(64383) |
安全信息
Pictograms【象形图】 | GHS07 |
Signal word【警示用语:】 | Warning |
Hazard Statements | H319 |
Precautionary Statements | P264 - P280 - P305 + P351 + P338 - P337 + P313 |
Hazard Classifications【危险分类】 | Eye Irrit. 2 |
Storage Class Code【储存分类代码】 | 10 - Combustible liquids |