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Sirtuin 2(SIRT2)抑制剂筛选测定试剂盒

Sirtuin 2 (SIRT2) Inhibitor Screening Assay Kit
产品编号:3240239
规格:100 assays in 96 well plates
包装规格:1 KIT
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
32402391 KIT8400
产品别名

Sirtuin 2(SIRT2)抑制剂筛选测定试剂盒

Sirtuin 2 (SIRT2) Inhibitor Screening Assay Kit

基本信息
NACRES
NA.41
General description【一般描述】
Sirtuin 2 (SIRT2) is a cytoplasmic protein and is part of the sirtuin family of proteins. Sirtuin family contains a sirtuin core domain and isgrouped into four classes with SIRT2 being a member of class I. SIRT2 colocalizes with microtubules. The gene encoding this protein is localized on human chromosome 19q13.2.

With Sigma′s Sirtuin Inhibitor Screening Kit, Sirtuin deacetylates the Substrate, and then a Developer cleaves the deacetylated substrate to release a fluorescent group, the latter of which can be detected at Ex/Em = 395/541 nm. In the presence of a SIRT inhibitor, the deacetylation will be impeded, preventing substrate cleavage and release of the fluorescent group. The kit provides a rapid, simple, sensitive, and reliable test, which is suitable for both low and high-throughput screening of SIRT2 inhibitors. A positive control inhibitor, Nicotinamide, is included to compare with the efficacy of the test inhibitors.
Application【应用】
Sirtuin 2 (SIRT2) inhibitor screening assay kit has been used for acetyl peptide deacetylase assay.
Biochem/physiol Actions【生化/生理作用】
Sirtuin 2 (SIRT2) maintains the integrity of the genome. Inhibition of SIRT2 can lead to neuroprotection in cellular and invertebrate models of Huntington′s disease. Huntington′s disease is characterized by increased sterol synthesis in neuronal cells and this process is reversed by SIRT2 inhibition. SIRT2 can deacetylate lys40 of α-tubulin both in vitro and in vivo. Knockdown of SIRT2 by small interfering RNA (siRNA) leads to tubulin hyperacetylation.
Features and Benefits【特点和优势】
  • Simple, sensitive, and reliable assay
  • Species reactivity: mammalian
  • Utilizes fluorometric methods
  • Suitable for individual tests or high throughput assays
  • Convenient 96-well microplate format
  • Screening and characterization of Sirtuin inhibitors
产品性质
usage【用途】
100 assays in 96 well plates
storage condition【储存条件】
protect from light
NCBI accession no.【NCBI登记号】
NM_001122765 (Mouse)
NM_001193286 (Human)
shipped in【运输】
wet ice
storage temp.【储存温度】
−20℃
Gene Information
human ... SIRT2(22933)
mouse ... SIRT2(64383)
安全信息
Pictograms【象形图】
GHS07
Signal word【警示用语:】
Warning
Hazard Statements
H319
Precautionary Statements
P264 - P280 - P305 + P351 + P338 - P337 + P313
Hazard Classifications【危险分类】
Eye Irrit. 2
Storage Class Code【储存分类代码】
10 - Combustible liquids
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