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Anti-Tensin 3 antibody, Mouse monoclonal
产品编号: | 3153382 |
规格: | clone TN-17, purified from hybridoma cell culture |
包装规格: | 200 μL |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
产品别名
Anti-Tensin 3 antibody, Mouse monoclonal
Monoclonal Anti-TEM6
Monoclonal Anti-TNS3
Monoclonal Anti-TENS1
Monoclonal Anti-Tensin 3 antibody produced in mouse
基本信息
NACRES | NA.41 |
General description【一般描述】 | Monoclonal Anti-Tensin 3 (mouse IgG1 isotype) is derived from the hybridoma TN-17 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to an internal sequence of human Tensin 3. Tensins are multidomain proteins that are characterized with homologous C1, protein phosphatases (PTPases) , C2, Src homology 2 (SH2) domains and pTyr-binding (PTB) domains. Tensin 3 (TNS3) is encoded by the gene mapped to human chromosome 7p12.3. TNS3 transcript is expressed at high level in thyroid and at moderate level in placenta. In addition, the protein is also expressed at minimal levels in brain, heart, lung, liver, pancreas, kidney, skeletal muscle, white blood cells and prostate. TNS3 is a member of tensin protein family. |
Immunogen【免疫原】 | synthetic peptide corresponding to an internal sequence of human Tensin 3, conjugated to KLH. The isotype is determined using a double diffusion immunoassay using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2). |
Application【应用】 | Monoclonal Anti-Tensin 3 antibody produced in mouse has been used in western blotting. Monoclonal Anti-Tensin 3 antibody produced in mouse has also been used in immunofluorescence. |
Biochem/physiol Actions【生化/生理作用】 | Tensin 3 (TNS3) plays a vital role in stimulation of epidermal growth factor (EGF) signaling pathway. The encoded protein supports fundamental role of thyroid hormone in normal thyroid tissues. Decreased expression of the gene leads to development of human renal cell carcinoma (RCC). Tensin 3 facilitates cell migration and tumorigenesis in cell lines from advanced lung cancer, breast cancer and melanoma. In these cell lines, The protein is phosphorylated by Src in its SH2 domain, leading to tumorigenesis and metastasis, implicating tensin 3 as an oncoprotein regulated by Src. |
Physical form【外形】 | 0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。 |
产品性质
Quality Level【质量水平】 | 200 |
biological source【生物来源】 | mouse |
conjugate【偶联物】 | unconjugated |
antibody form【抗体形式】 | purified from hybridoma cell culture |
antibody product type | primary antibodies |
clone【克隆】 | TN-17, monoclonal |
form【形式】 | buffered aqueous solution |
mol wt【分子量】 | antigen ~170 kDa |
species reactivity | human |
concentration【浓度】 | ~1.0 mg/mL |
technique(s) | indirect immunofluorescence: 2.5-5.0 μg/mL using PC3 cells. western blot: 0.5-1.0 μg/mL using extracts of SW-620 cells. |
isotype【同位素/亚型】 | IgG1 |
UniProt accession no.【UniProt登记号】 | Q68CZ2 |
shipped in【运输】 | dry ice |
storage temp.【储存温度】 | −20℃ |
Gene Information | human ... TNS3(64759) |
产品说明
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |