Monoclonal Anti-SPAG11 antibody produced in mouse

NA.41

SPAG11A (sperm associated antigen 11A) belongs to the β defensin family. The protein is mainly expressed in the epididymis. The gene is mapped to human chromosome 8.
Monoclonal Anti-SPAG11 (mouse IgG1 isotype) is derived from the hybridoma 10B4 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. SPAG11 is expressed specifically in epithelial cells in the middle part of the caput region. It exists in four isoforms such as A, D, E and G.

Monoclonal Anti-SPAG11 recognizes mouse and rat SPAG11.

a recombinant rat SPAG11

Monoclonal Anti-SPAG11 antibody produced in mouse may be used in immunoblotting and immunohistochemistry.

SPAG11A (sperm associated antigen 11A) is a secretory protein with possible involvement in sperm maturation. It also controls inflammatory responses in the epididymis.
SPAG11 is involved in promoting sperm motility due to its capability to bind the sperm head in different regions of the epididymis. It exhibits anti-microbial activity by suppressing colony growth of Escherichia coli and other Gram-negative bacteria.

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

200

mouse

purified immunoglobulin

primary antibodies

10B4, monoclonal

buffered aqueous solution

monomer ~7 kDa
~34 kDa (multimer)

mouse, rat

~1 mg/mL

immunoblotting: 4-8 μg/mL using whole extracts of HEK-293 cells overexpressing rat SPAG11
immunohistochemistry: 5-10 μg/mL using paraffin-embedded rat caput epididimys sections

IgG1

dry ice

−20℃

mouse ... Spag11a(78128)
rat ... Spag11a(246305)

For extended storage, freeze at -20 ℃ in working aliquots. Repeated freezing and thawing or storage in “frost-free” freezer is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

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12 - Non Combustible Liquids