Monoclonal Anti-p120^ctn (Catenin-related) antibody produced in mouse

MFCD02097107

NA.41

Monoclonal Anti-p120ctn (Catenin-related) (mouse IgG1 isotype) is derived from the 6H11 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an A/J mouse immunized with a recombinant N-terminal fragment of murine p120ctn. Catenin δ-1 (p120ctn) contains a central armadillo repeat domain (ARM domain) and is structurally similar to β-catenin and plakoglobin. At least six alternatively spliced p120ctn isoforms are generated and differentially expressed in different cell types.

Reacts specifically with the N-terminal region of p120^ctn and recognizes isoforms 1 and 2, but not isoforms 3 or 4. In immunoblotting, the antibody recognizes a 120 kDa band, and an additional very weak doublet at 200 kDa.

recombinant N-terminal fragment of murine p120^ctn.

Monoclonal Anti-p120ctn (Catenin-related) antibody produced in mouse has been used in:

• immunoblotting
• enzyme linked immunosorbent assay (ELISA),
• immunocytochemistry
• immunoprecipitation

The 120 kD catenin-related protein p120ctn (also known as catenin p120ctn, p120cas and pp120 src substrate), was originally described as a prominent substrate for the sarcoma (Src) oncoprotein and for a variety of receptor tyrosine kinases (RTKs) including those for epidermal growth factor (EGF), platelet derived growth factor (PDGF), and colony stimulating factor-1 (CSF1). Like β-catenin and plakoglobin, it binds directly to the cytoplasmic domain of epithelial (E)-cadherin via the ARM domain and co-precipitates in multiprotein complexes containing E-cadherin or other cadherins such as neural (N)- or placental (P)- cadherins, depending on the cell type. However, unlike β-catenin and plakoglobin, p120ctn does not appear to bind α-catenin.

200

mouse

unconjugated

tissue culture supernatant

primary antibodies

6H11, monoclonal

15 mM sodium azide

rat, chicken, canine, human, mouse, bovine

immunoprecipitation (IP): suitable
indirect ELISA: suitable
indirect immunofluorescence: 1:100 using cultured Madin Darby canine kidney (MDCK) epithelial cells
western blot: 1:10,000 using whole cell extract of cultured chicken fibroblasts

IgG1

O60716

dry ice

−20℃

human ... CTNND1(1500)
mouse ... Ctnnd1(12388)
rat ... Ctnnd1(311163)

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12 - Non Combustible Liquids

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