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Monoclonal Anti-MAP Kinase, Activated/monophosphorylated (Phosphothreonine ERK-1&2) antibody produced in mouse
| 产品编号： | 3148102 |
| 规格： | ~2 mg/mL, clone ERK-PT115, purified immunoglobulin, buffered aqueous solution |
| 包装规格： | 0.2 ML |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
基本信息
| MDL number | MFCD01633612 |
| NACRES | NA.44 |
| General description【一般描述】 | Activated/ Monophosphorylated (Phosphothreonine ERK-1&2) (mouse IgG1 isotype) is derived from the ERK-PT115 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. Mitogen-activated protein kinase 1 (MAPK1) is mapped to human chromosome 22q11.22 and MAPK3 is present on human chromosome 16p11.2. |
| Specificity【特异性】 | The antibody reacts specifically with the monophosphorylated threonine and the active diphosphorylated forms of MAP kinase (ERK-1 and ERK-2). It does not recognize the non-phosphorylated form of the MAP kinase molecule, or the diphosphorylated forms of JNK and p38 MAPK. The epitope recognized by the antibody contains the phosphorylated threonine residue within the regulatory site of active MAP kinase (e.g.,Thr183 in ERK-2). |
| Immunogen【免疫原】 | synthetic peptide HTGFLpTEYVAT, corresponding to the phosphorylated form of ERK-activation loop. |
| Application【应用】 | Monoclonal Anti-MAP Kinase, Activated/monophosphorylated (Phosphothreonine ERK-1&2) antibody produced in mouse has been used in:
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| Physical form【外形】 | 0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。 |
产品性质
| Quality Level【质量水平】 | 200 |
| biological source【生物来源】 | mouse |
| conjugate【偶联物】 | unconjugated |
| antibody form【抗体形式】 | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone【克隆】 | ERK-PT115, monoclonal |
| form【形式】 | buffered aqueous solution |
| mol wt【分子量】 | antigen, ERK-1 44 kDa antigen, ERK-2 42 kDa |
| species reactivity | human, rat |
| concentration【浓度】 | ~2 mg/mL |
| technique(s) | capture ELISA: suitable immunocytochemistry: suitable microarray: suitable western blot: 0.5 μg/mL using extract of a rat fibroblast cell line, Rat1, activated with sorbitol |
| isotype【同位素/亚型】 | IgG1 |
| UniProt accession no.【UniProt登记号】 | P28482 |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −20℃ |
| Gene Information | human ... MAPK1(5594), MAPK3(5595) rat ... Mapk1(116590), Mapk3(50689) |
产品说明
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | nwg |
