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Monoclonal Anti-μ-Calpain (Domain III) antibody produced in mouse
产品编号: | 3148017 |
规格: | clone 9A4H8D3, ascites fluid, buffered aqueous solution |
包装规格: | 100 μL |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
基本信息
MDL number | MFCD01322347 |
NACRES | NA.41 |
General description【一般描述】 | Calpain-1 catalytic subunit is a protein encoded by the CAPN1 gene in humans. Calpains are calcium dependent proteases constituting a family of proteins. They share a homologous cysteine-protease domain and an E-F hand Ca2+-binding domain. The calpain system consists of two ubiquitous forms of calpain (m-calpain and μ-calpain), a tissue specific calpain (n-calpain) and a calpain inhibitory protein (calpastatin). |
Specificity【特异性】 | Epitope mapping studies indicate the epitope is between amino acids 465-520 (domain III) of human μ-calpain. The antibody reacts specifically with μ-calpain. It does not cross-react with m-calpain, n-calpain, calmodulin or calpastatin. It is not recommended for immunoprecipitation. By immunoblotting, reactivity is observed with human platelets and erythrocytes, bovine platelets, heart and skeletal muscle and with rat myoblasts, kidney, liver and spleen. By immunofluorescence on pig LLC-PK1 cells, diffuse cytoplasmic staining is observed. |
Immunogen【免疫原】 | μ-calpain from bovine skeletal muscle. |
Application【应用】 | Monoclonal Anti-μ-Calpain (Domain III) antibody produced in mouse is suitable for indirect immunofluorescence at a dilution of 1:100 and western blotting at a dilution of 1:2,000. |
Biochem/physiol Actions【生化/生理作用】 | Calpain is involved in calmodulin-independent pathway for the activation of calcineurin. Endogenous calpain I forms active calcineurin in the human heart by proteolysis of calcineurin A, which may lead to pathogenesis of myocardial disease. μ-calpain, on overexpression, may have relationship with intractable epilepsy as well as the clinicopathological characteristics. The activity of this protein is elevated in skeletal muscle from gastric cancer patients. |
Physical form【外形】 | Solution in phosphate buffered saline and contains 0.05% sodium azide. |
产品性质
Quality Level【质量水平】 | 200 |
biological source【生物来源】 | mouse |
conjugate【偶联物】 | unconjugated |
antibody form【抗体形式】 | ascites fluid |
antibody product type | primary antibodies |
clone【克隆】 | 9A4H8D3, monoclonal |
form【形式】 | buffered aqueous solution |
mol wt【分子量】 | antigen 80 kDa |
species reactivity | rat, pig, human, bovine |
technique(s) | indirect immunofluorescence: 1:100 western blot: 1:2,000 |
isotype【同位素/亚型】 | IgG1 |
UniProt accession no.【UniProt登记号】 | P07384 |
shipped in【运输】 | dry ice |
storage temp.【储存温度】 | −20℃ |
Gene Information | human ... CAPN1(823) rat ... Capn1(29153) |
产品说明
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | nwg |
Personal Protective Equipment【个人防护装备】 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |