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Monoclonal Anti-hnRNP-K/J antibody produced in mouse

产品编号:3146502
规格:clone 3C2, purified immunoglobulin, buffered aqueous solution
包装规格:200 μL
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
3146502200 μL5040
产品别名

Monoclonal Anti-hnRNP-K/J antibody produced in mouse

Anti-Heterogeneous Nuclear Ribonucleoprotein K/J

基本信息
MDL number
MFCD06798704
NACRES
NA.41
General description【一般描述】
Monoclonal Anti-hnRNP-K/J (mouse IgG2b isotype) is derived from the 3C2 hybridoma produced by the fusion of mouse myeloma cells (SP2/0 cells) and splenocytes from BALB/c mice immunized with hnRNP′s purified by oligo (dC) affinity chromatography.
Immunogen【免疫原】
hnRNPs purified by oligo (dC) affinity chromatography.
Application【应用】
The antibody may be used in various immunochemical techniques including enzyme linked immunosorbent assay (ELISA), immunoblotting, chromatin immunoprecipitation (ChIP), immunocytochemistry, antibody-based supershift assay and electrophoretic mobility shift assay (EMSA).
Biochem/physiol Actions【生化/生理作用】
Heterogeneous nuclear ribonucleoproteins (hnRNPs) act in several biological activities such as transcription, pre- mRNA processing, cytoplasmic mRNA translation and turnover. hnRNP-K is found both in the nucleus, cytoplasm, and mitochondria, therefore, it was found to be involved in many biological events in the cell like: transcription, chromatin remodeling, splicing and translation. hnRNP-K is regulated by different signaling cascades and can bind kinases. As a consequence, it can mediate signals from the cytoplasm to the nucleus.
Physical form【外形】
0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。
产品性质
Quality Level【质量水平】
200
biological source【生物来源】
mouse
conjugate【偶联物】
unconjugated
antibody form【抗体形式】
purified immunoglobulin
antibody product type
primary antibodies
clone【克隆】
3C2, monoclonal
form【形式】
buffered aqueous solution
mol wt【分子量】
antigen ~60 kDa
species reactivity
bovine, Xenopus, hamster, human
technique(s)
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 0.1-0.2 μg/mL using total cell extract of MBDK cells
isotype【同位素/亚型】
IgG2b
UniProt accession no.【UniProt登记号】
P61978
shipped in【运输】
dry ice
storage temp.【储存温度】
−20℃
Gene Information
human ... HNRNPK(3190)
产品说明
Disclaimer【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
安全信息
Storage Class Code【储存分类代码】
12 - Non Combustible Liquids
WGK
nwg
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