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Monoclonal Anti-Histone Deacetylase 8 (HDAC8) antibody produced in mouse
| 产品编号： | 3146368 |
| 规格： | 2.0-2.5 mg/mL, clone HDAC8-48, purified immunoglobulin, buffered aqueous solution |
| 包装规格： | 200 μL |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
基本信息
| MDL number | MFCD04975119 |
| NACRES | NA.41 |
| General description【一般描述】 | Monoclonal Anti-Histone Deacetylase 8 (HDAC8) (mouse IgG1 isotype) is derived from the HDAC8-48 hybridoma produced by the fusion of NS-1 mouse myeloma cells and splenocytes from BALB/c mice immunized with recombinant human HDAC8. Histone deacetylases (HDACs) are competing enzymes, belonging to histone deacetylase family. There are two classes of HDACs with six to seven different types of HDACs proteins. HDAC1,HDAC2, HDAC3, and HDAC8 belong to Class I HDACs and HDAC4, HDAC6, HDAC7, HDAC9, and HDAC10 belong to Class II HDACs. Class I HDACs consists of a single deacetylase domain at the N-termini and diversified C-terminal regions, while Class II contains a deacetylase domain at C-terminal position. Studies show that HDAC8 is a sex-linked gene located on the chromosome at position Xq21.2 - q21.3. HDAC8 gene has a molecular weight of 43kDa and it encodes a 377 amino acid protein. It is present within the nucleas. HDAC8 mRNA is seen in heart, lung, kidney, and pancreas. |
| Immunogen【免疫原】 | recombinant human HDAC8 |
| Application【应用】 | Monoclonal Anti-Histone Deacetylase 8 (HDAC8) antibody produced in mouse has been used in :
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| Biochem/physiol Actions【生化/生理作用】 | Histone deacetylation results in transcription repression leading to the formation of tight nucleosomal structure which prevents DNA accessing. HDAC8 controls HDAC activity on H4 histone peptide substrates. HDAC8 is similar to the HDAC class I enzymes. In vitro expression and activity of HDAC8 was examined using FLAG tagged- HDAC8 and HDAC1. These were transfected into HeLa cells and Sf9 insect cells. After the immunoprecipitation of cell lysates the expression results were confirmend using Western blotting. Studies show that HDAC8 may play a role in transcriptional regulation and could possibly be regulated in a temporal or compartment-specific manner. |
| Physical form【外形】 | 0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。 |
产品性质
| Quality Level【质量水平】 | 200 |
| biological source【生物来源】 | mouse |
| conjugate【偶联物】 | unconjugated |
| antibody form【抗体形式】 | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone【克隆】 | HDAC8-48, monoclonal |
| form【形式】 | buffered aqueous solution |
| mol wt【分子量】 | antigen ~43 kDa |
| species reactivity | human |
| concentration【浓度】 | 2.0-2.5 mg/mL |
| technique(s) | indirect ELISA: suitable microarray: suitable western blot: 4 μg/mL using nuclear extracts of HeLa cells |
| isotype【同位素/亚型】 | IgG1 |
| UniProt accession no.【UniProt登记号】 | Q9BY41 |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −20℃ |
| Gene Information | human ... HDAC8(55869) |
| packaging【包装】 | 200 μL in glass bottle |
产品说明
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
