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Anti-hABH3 antibody, Mouse monoclonal
| 产品编号： | 3146131 |
| 规格： | clone hABH3-99, purified from hybridoma cell culture |
| 包装规格： | 200 μL |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Anti-hABH3 antibody, Mouse monoclonal
Anti-Human AlkB Homologue 3
Monoclonal Anti-hABH3 antibody produced in mouse
基本信息
| MDL number | MFCD06412252 |
| NACRES | NA.41 |
| General description【一般描述】 | Human alkB homolog 3, alpha-ketoglutarate dependent dioxygenase (hABH3) is mainly found in the nucleoplasm and cytoplasm. Anti-hABH3 antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hABH3-99 hybridoma produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with hABH3, conjugated to keyhole limpet hemocyanin (KLH). |
| Specificity【特异性】 | Mouse Monoclonal Anti-hABH3 binds to human, monkey, bovine, dog, rat, hamster, and mouse hABH3. It does not recognize hABH1 or hABH2. The antibody epitope resides within amino acids 2-16 of human hABH3. |
| Application【应用】 | Mouse Monoclonal Anti-hABH3 can be used for western blot (1-2μg/mL), immunohistochemistry, immunoprecipitation, indirect ELISA and microarray studies.
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| Biochem/physiol Actions【生化/生理作用】 | Human alkB homolog 3, α-ketoglutarate dependent dioxygenase (hABH3) is involved in the repair of alkylated RNA. This antibody serves as an important tool for studying the regulation of DNA/RNA repair enzymes. hABH3 is a Fe(II)-dependent dioxygenase involved in the repair of alkylation-induced damage in DNA. hABH3 mutations have been analysed in pediatric brain tumors. Mouse Monoclonal Anti-hABH3 binds to human, monkey, bovine, dog, rat, hamster, and mouse hABH3. It does not recognize hABH1 or hABH2. The antibody epitope resides within amino acids 2-16 of human hABH3. |
| Physical form【外形】 | Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide. |
产品性质
| Quality Level【质量水平】 | 200 |
| biological source【生物来源】 | mouse |
| conjugate【偶联物】 | unconjugated |
| antibody form【抗体形式】 | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone【克隆】 | hABH3-99, monoclonal |
| form【形式】 | buffered aqueous solution |
| mol wt【分子量】 | antigen ~33 kDa |
| species reactivity | monkey, bovine, mouse, hamster, human, canine, rat |
| technique(s) | immunohistochemistry: suitable immunoprecipitation (IP): suitable indirect ELISA: suitable microarray: suitable western blot: 1-2 μg/mL using total cell extract of 293T cells |
| isotype【同位素/亚型】 | IgG1 |
| UniProt accession no.【UniProt登记号】 | Q96Q83 |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −20℃ |
| Gene Information | Monkey ... ALKBH3(715921) human ... ALKBH3(221120) mouse ... Alkbh3(69113) rat ... Alkbh3(362169) |
产品说明
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
