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抗 砂砾素抗体，小鼠单克隆
Anti-Gravin antibody, Mouse monoclonal
| 产品编号： | 3145905 |
| 规格： | clone JP74, purified from hybridoma cell culture |
| 包装规格： | 200 μL |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
抗 砂砾素抗体，小鼠单克隆
Anti-Gravin antibody, Mouse monoclonal
Anti-AKAP12
Anti-A-Kinase Anchor Protein 12
基本信息
| MDL number | MFCD08705585 |
| NACRES | NA.44 |
| General description【一般描述】 | Monoclonal Anti-Gravin (mouse IgG2a isotype) is derived from the hybridoma JP74 produced by the fusion of mouse myeloma cells (X63Ag8.653) and splenocytes from BALB/c mice immunized with fractionated membrane cortex proteins from human neurons [cell lines NT2-N and SK-N-BE(2)]. Gravin protein is a myristoylated high molecular weight cytoplasmic protein expressed in endothelial cells, cultured fibroblasts and osteosarcoma cells and highly expressed in the brain. |
| Specificity【特异性】 | Monoclonal Anti-Gravin antibody is specific for human, rat and mouse gravin (approx. 300 kDa). |
| Immunogen【免疫原】 | fractionated membrane cortex proteins from human neuron cell lines NT2-N and SK-N-BE(2). |
| Application【应用】 | Anti-Gravin antibody has been used in western blotting immunofluorescence. Monoclonal Anti-Gravin antibody is suitable for use in immunoblotting (using mpkCCD cells ). The product may also be used for immunocytochemistry and immunoprecipitation. |
| Biochem/physiol Actions【生化/生理作用】 | Gravin (or AKAP12) is a scaffolding kinase that functions as a tumor suppressor. This kinase interacts with PKC, PKA and other cell cycle signaling proteins to modulate cell division, growth, differentiation and inflammatory responses. The expression of gravin isoforms (α, β, and γ) is regulated by tissue-specific promoters. Gravin also acts as an autoantigen in myasthenia gravis patients . |
| Physical form【外形】 | 0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。 |
产品性质
| Quality Level【质量水平】 | 200 |
| biological source【生物来源】 | mouse |
| conjugate【偶联物】 | unconjugated |
| antibody form【抗体形式】 | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone【克隆】 | JP74, monoclonal |
| form【形式】 | buffered aqueous solution |
| species reactivity | rat, human, mouse |
| technique(s) | immunocytochemistry: suitable immunoprecipitation (IP): suitable western blot: 0.02-0.04 μg/mL using rat brain cytosolic S1 fraction |
| isotype【同位素/亚型】 | IgG2a |
| UniProt accession no.【UniProt登记号】 | Q02952 |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −20℃ |
| Gene Information | human ... AKAP12(9590) mouse ... Akap12(83397) |
产品说明
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 3 |
| Personal Protective Equipment【个人防护装备】 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |
