Monoclonal Anti-Episialin (EMA) antibody produced in mouse

MFCD00211919

NA.46

Monoclonal Anti-Episialin (EMA) (mouse IgG1 isotype) is derived from the GP1.4 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.
Episialin (mucin-1) is an epithelial transmembrane oncoprotein that occurs in glandular tissues. This oncoprotein mediates ErbB1 receptor transport and subsequently regulates cell survival. Episialin also modulates p53-responsive genes under conditions of genotoxic stress and also co-activates the transcription of Wnt target genes. Overexpression of Episialin has been associated with most human cancers.

human milk fat globule membranes.

Cell-membrane-associated, mucin-like molecules, such as episialin has a protective function against toxic substances. Data on the effect of episialin on the adhesion properties of cultured cells suggests that episialin expressed at high levels on cells may have a major function in reducing the aggregation capacity of these cells, thus influencing the adherence to various extracellular matrix components by masking adhesion molecules.
Monoclonal Anti-Episialin (EMA) antibody is suitable for use in immunohistochemistry (1:200 using formalin-fixed, paraffin-embedded sections of human breast carcinoma), immunoprecipitation and microarray.

Episialin is a transmembrane, high molecular weight, mucin-like glycoprotein (apparent molecular weight of 265-400 kDa) containing many carbohydrate side chains, that are predominantly attached to the molecule by O-glycosidic linkages. It is known as epithelial membrane antigen (EMA), MUC1 and polymorphic epithelial mucin (PEM). The episialin molecule is transmembranous with a relatively large extracellular domain and a cytoplasmic domain of 69 amino acids. The extracellular domain consists mainly of a region of nearly identical repeats of 20 amino acids, the number of which can vary between about 30 and 90 because of genetic polymorphism. The episialin molecule is synthesized as one large precursor containing only N-linked glycans and is immediately proteolytically cleaved while still in the endoplasmic reticulum.

200

mouse

unconjugated

ascites fluid

primary antibodies

GP1.4, monoclonal

15 mM sodium azide

human

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using formalin-fixed, paraffin-embedded sections of human breast carcinoma
immunohistochemistry (frozen sections): suitable
immunoprecipitation (IP): suitable
microarray: suitable

IgG1

P15941

dry ice

−20℃

human ... MUC1(4582)

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12 - Non Combustible Liquids

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