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Anti-E2F3 antibody ,Mouse monoclonal

产品编号:3144358
规格:clone PG30, purified from hybridoma cell culture
包装规格:0.2 ML
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
31443580.2 ML4730
基本信息
MDL number
MFCD00283127
NACRES
NA.41
General description【一般描述】
Monoclonal Anti-E2F3 (mouse IgG2a isotype) is derived from the PG30 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a recombinant human E2F Transcription Factor 3 (E2F3) protein. Transcription factor E2F3 is a DNA-binding protein of ~57 kDa encoded by the E2F3 gene in humans. It belongs to E2F transcription factor family of proteins that bind to the sequence TTTCGCGC.
Immunogen【免疫原】
recombinant human E2F3.
Application【应用】
Monoclonal Anti-E2F3 antibody produced in mouse has been used for the following applications:
  • immunoprecipitation using denatured E2F3
  • western blotting at a concentration of 4-8μg/mL using transfected 293T cells expressing E2F3
  • electrophoretic mobility shift assay (EMSA).
Biochem/physiol Actions【生化/生理作用】
Transcription factor E2F3 is involved in the regulation of cell cycle. E2F3 helps in the regulation of the expression of a number of genes that are important in cell proliferation. It is a oncogene with strong proliferative potential and is involved in many processes. E2F3 plays an important role in the development of several types of cancer. Overexpression of E2F3 may be associated with hepatocellular carcinoma (HCC). It plays an important role in controlling cell cycle progression and proliferation. E2F3 is frequently expressed in pediatric Wilms′ tumors and may be associated with the same.
Physical form【外形】
Solution in phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.
Preparation Note【制备说明】
Purified from culture supernatant of hybridoma cells grown in a bioreactor.
产品性质
Quality Level【质量水平】
200
biological source【生物来源】
mouse
conjugate【偶联物】
unconjugated
antibody form【抗体形式】
purified from hybridoma cell culture
antibody product type
primary antibodies
clone【克隆】
PG30, monoclonal
form【形式】
buffered aqueous solution
mol wt【分子量】
antigen 57 kDa
species reactivity
human
concentration【浓度】
~2 mg/mL
technique(s)
immunoprecipitation (IP): suitable using denatured E2F3
microarray: suitable
western blot: 4-8 μg/mL using transfected 293T cells expressing E2F3
isotype【同位素/亚型】
IgG2a
UniProt accession no.【UniProt登记号】
O00716
shipped in【运输】
dry ice
storage temp.【储存温度】
−20℃
Gene Information
human ... E2F3(1871)
产品说明
Disclaimer【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
安全信息
Storage Class Code【储存分类代码】
12 - Non Combustible Liquids
WGK
nwg
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