Anti-DNA polymerase δ p125 antibody, Rat monoclonal

NA.41

Monoclonal Anti-DNA polymerase d p125 (rat IgG2a isotype) is derived from the hybridoma PDG 5G1 produced by the fusion of mouse myeloma cells (P3X63Ag8.653) and splenocytes from rat immunized with human p125. The catalytic subunit p125 (POLD1), which is encoded by the Pold1 gene, is highly conserved among eukaryotes. Pold1 comprises of two primary functional domains: an exonuclease domain near the N-terminus and the subsequent polymerase domain.

human p125

Monoclonal Anti-DNA polymerase δ p125 antibody produced in rat has been used in:

• immunoblotting
• immunoprecipitation
• immunocytochemistry
• co-immunoprecipitation

The exonuclease domain of the catalytic subunit p125 (POLD1) has the ability to catalyze 3′−5′ exonucleolytic proofreading and the polymerase domain helps to catalyze DNA synthesis. Mutations in this subunit have been implicated in mammalian embryogenesis and tumorigenesis.

Solution in 0.01M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

200

rat

purified from hybridoma cell culture

primary antibodies

PDG 5G1, monoclonal

buffered aqueous solution

antigen ~125 kDa

human, mouse, rat

antibody small pack of 25 μL

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: 0.5-1.0 μg/mL using Jurkat cells extract

P28340

dry ice

−20℃

human ... POLD1(5424)

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