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Monoclonal Anti-Dihydropyridine Receptor (α2 Subunit) antibody produced in mouse
| 产品编号： | 3144132 |
| 规格： | clone 20A, ascites fluid, buffered aqueous solution |
| 包装规格： | 100 μL |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
基本信息
| MDL number | MFCD01323189 |
| NACRES | NA.41 |
| General description【一般描述】 | Dihydropyridine Receptor (DHPR) is a heteropentamer calcium ion channel and comprises of α1,α2/δ,β and γ subunits. It is localized in the skeletal muscle in the t-tubule membrane. The α2subunit of DHPR is a glycosylated subunit and is anchored on the membrane. |
| Specificity【特异性】 | Reacts specifically with the α2 subunit of the DHP receptor. Strong reactivity is observed with the α2 subunit in rat, mouse, guinea pig, rabbit skeletal and cardiac muscle, and rat brain and lung tissue. Weaker reactivity is observed with rat kidney, liver and spleen tissues. By immunohistochemistry, double rows of discrete punctate staining represent pairs of triads on the opposing sides of the Z-lines. |
| Immunogen【免疫原】 | Rabbit dihydropyridine (DHP) receptors, (α2 subunit) |
| Application【应用】 | Use of this antibody for immunoblot analysis will allow for detection of a 220 kDa band (native conditions) or a 143 kDa band (denaturing conditions).Use of this antibody for immunohistochemistry of muscle tissue will demonstrate double rows of discrete punctate staining representing pairs of triads on the opposing sides of the Z-lines are observed. Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Western Blotting (1 paper) Monoclonal Anti-Dihydropyridine Receptor (α2 Subunit) antibody produced in mouse has been used in:
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| Biochem/physiol Actions【生化/生理作用】 | Dihydropyridine Receptor (DHFR) α1 subunit is the voltage-sensor. DHPR interacts with ryanodine receptor (RyR) and also mediates excitation-contraction (EC) coupling in cardiac and skeletal muscles. Mutation in the DHPR (E1014K) affects its affinity and selectivity towards calcium permeation. Additionally, it also plays a key role in the synaptic transmission. |
| Physical form【外形】 | Solution in phosphate buffered saline containing 0.05% sodium azide. |
| Other Notes【其他说明】 | This mouse monoclonal IgG2a antibody is supplied as a mouse ascites diluted in phosphate buffered saline containing 0.05% sodium azide as a preservative. |
产品性质
| Quality Level【质量水平】 | 200 |
| biological source【生物来源】 | mouse |
| conjugate【偶联物】 | unconjugated |
| antibody form【抗体形式】 | ascites fluid |
| antibody product type | primary antibodies |
| clone【克隆】 | 20A, monoclonal |
| form【形式】 | buffered aqueous solution |
| mol wt【分子量】 | antigen 143 kDa (reduced) antigen 220 kDa (non-reduced) |
| species reactivity | guinea pig, rabbit, mouse, rat |
| technique(s) | immunohistochemistry (frozen sections): 1:500 western blot: 1:500 |
| isotype【同位素/亚型】 | IgG2a |
| UniProt accession no.【UniProt登记号】 | O08532 |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −20℃ |
| Gene Information | mouse ... Cacna2d1(12293) rat ... Cacna2d1(25399) |
产品说明
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | nwg |
| Personal Protective Equipment【个人防护装备】 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |
