Anti-Cyclophilin B antibody, Mouse monoclonal

NA.41

Monoclonal Anti-Cyclophilin B (mouse IgG1 isotype) is derived from the hybridoma CYPB-3 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to the C-terminal region of human Cyclophilin B, conjugated to keyhole limpet hemocyanin (KLH). Cyclophilin B (CypB) is a 21 kDa protein encoded by the peptidylprolyl isomerase B (PPIB) gene that belongs to the cyclophilin family, is a highly conserved class of proteins originally identified as cellular binding proteins for the immunosuppressive drug cyclosporin A. CypB is mainly located within the endoplasmic reticulum (ER) of all cell types.
Cyclophilin B belongs to the cyclophilin family of immunophilins. It is present in the cytoplasm, nucleus, endoplasmic reticulum (ER)/golgi and plasma membrane. The protein can also be secreted from cells. The associated gene is mapped to human chromosome 15q22.

synthetic peptide corresponding to the C-terminal region of human Cyclophilin B, conjugated to KLH. The corresponding sequence is identical in mouse, monkey and canine and differs by one amino acid in rat Cyclophilin B.

Monoclonal Anti-Cyclophilin B antibody produced in mouse has been used in immunoblotting and immunofluorescence.

In endoplasmic reticulum (ER), Cyclophilin B works as a chaperone for exported proteins. It also participates in the intracellular transport of collagen and proper formation of collagen fibril. Cyclophilin B can bind to the Gag protein of human immunodeficiency type 1 (HIV-1) and helps in HIV replication. It is also involved in the infection of hepatitis C virus (HCV), Japanese encephalitis virus (JEV) and human papillomavirus type 16 (HPV16). Cyclophilin B protects against ER-mediated hypoxia-induced cell death. It is overexpressed in various malignancies.
Cyclophilin B (CypB) is involved in inflammation, viral infection and cancer. It also plays an important role in protecting cells against endoplasmic reticulum (ER) stress via its PPIase activity. Mutations in the PPIB gene give rise to recessive forms of osteogenesis imperfecta and knockdown of this gene decreases cell growth, proliferation and migration.

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

200

mouse

unconjugated

purified from hybridoma cell culture

primary antibodies

CYPB-3, monoclonal

buffered aqueous solution

antigen ~20 kDa

mouse, human, rat

~1.0 mg/mL

western blot: 2.5-5.0 μg/mL using whole cell extracts of human HepG2 or rat NRK cells.

IgG1

P23284

dry ice

−20℃

human ... PPIB(5479)
mouse ... Ppib(19035)
rat ... Ppib(64367)

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12 - Non Combustible Liquids