Anti-Coilin antibody, Mouse monoclonal antibody produced in mouse

MFCD03792415

NA.41

Monoclonal Anti-Coilin (mouse IgG1 isotype) is derived from the pδ hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with coilin. Coilin contains two nuclear localization sequences (NLS) (at amino acid 107-112 and 181-198) and several serine residues that are phosphorylated in vivo. The description of specific intranuclear structures known today as Cajal bodies was first published in 1903 by the neuro-cytologist Ramon-γ-Cajal who discovered that neurons contained spherical structures of around 0.5 μm in diameter that were often associated with nucleoli, nucleolar accessory bodies. It was found that patients with auto-antibodies against coiled bodies recognize a protein of 80 kDa termed p80-coilin. Nuclear antigens shown to colocalize with p80 coilin in Cajal bodies include basal transcription factors, cell cycle factors (cdks), splicing snRNPs and nucleolar factors including snoRNP.

The antibody recognizes the C-terminal region of human coilin and does not recognize mouse coilin.

C-terminal (389 amino acids) human coilin

Monoclonal Anti-Coilin antibody produced in mouse has been used in:

• immunoblotting
• immunocytochemistry
• cell microinjection
• fluorescence imaging
• indirect immunofluorescence

Mutating Serine-202 to Aspartate causes the disappearance of coiled bodies and a redistribution of coilin to intranucleolar domains. Coilin plays a key role in ribonucleoprotein and Cajal body formation.

Supplied as a solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

200

mouse

unconjugated

purified from hybridoma cell culture

primary antibodies

pδ, monoclonal

buffered aqueous solution

antigen ~80 kDa by SDS-PAGE

human

~1.5 mg/mL

immunocytochemistry: suitable
microarray: suitable
western blot: 1-2 μg/mL using HeLa nuclear extract

IgG1

P38432

dry ice

−20℃

human ... COIL(8161)

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12 - Non Combustible Liquids

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