Monoclonal Anti-CD3 antibody produced in mouse

NA.41

The mouse monoclonal antibody APA1/1 recognizes an activation-dependent intracellular epitope of CD3 epsilon. Exposure of the epitope precedes CD3 phosphorylation and recruitment and activation of ZAP70, which initiates the signaling cascade produced by T-cell activation. APA1/1 provides the earliest known marker for TCR-mediated T cell activation.
CD3 (cluster of differentiation 3) molecule is encoded by the gene mapped to human chromosome 11q23.3. The encoded protein exists in three isoforms, CD3ε, CD3γ and CD3δ and each contains an N-terminal extracellular domain, a transmembrane segment and a cytoplasmic domain. CD3 is a 20kDa glycoprotein expressed on surface of all human T lymphocytes.

Purified human CD3 proteins isolated from thymus

The reagent is designed for Flow Cytometry analysis. Suggested working dilution for Flow Cytometry is 1-2 μg/mL of sample. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.

T-cell receptor- CD3 (cluster of differentiation 3) complex including CD3ε, CD3γ and CD3δ, plays a vital role in inducing early metabolic events that lead to T cell activation. Mutation in CD3ε has been observed in T-B+ NK+ severe combined immunodeficiency (SCID) patients.

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Solution in phosphate buffered saline, pH 7.4, with 15 mM sodium azide.

100

mouse

unconjugated

purified immunoglobulin

primary antibodies

APA1/1, monoclonal

buffered aqueous solution

mouse, human

1 mg/mL

flow cytometry: suitable

IgG1

NM_000733.3

wet ice

2-8℃

human ... CD3(916)

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12 - Non Combustible Liquids