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单克隆抗 c-Myc-FITC 小鼠抗

Monoclonal Anti-c-Myc-FITC antibody produced in mouse
产品编号:3141893
规格:clone 9E10, purified immunoglobulin, buffered aqueous solution
包装规格:100 μG
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
3141893100 μG4470
产品别名

单克隆抗 c-Myc-FITC 小鼠抗

Monoclonal Anti-c-Myc-FITC antibody produced in mouse

Anti-c-Myc

抗-c-Myc

基本信息
NACRES
NA.56
General description【一般描述】
Monoclonal Anti-c-Myc (mouse IgG1 isotype) is derived from the 9E10 hybridoma, produced by fusion of mouse myeloma cells and splenocytes from BALB/c mice. The antibody is isolated from ascites fluid and conjugated to fluorescein isothiocyanate isomer I (FITC). Monoclonal Anti-c-Myc, FITC conjugate recognizes the c-Myc tag sequence on c-Myc tagged fusion proteins when expressed N- or C-terminal to the fusion protein. c-Myc is a proto-oncogene localized on long arm of human chromosome 8. The encoded protein contains an N-terminal transactivation domain.
Specificity【特异性】
Monoclonal Anti-c-Myc, FITC conjugate recognizes the c-Myc tag sequence on c-Myc tagged fusion proteins when expressed N- or C-terminal to the fusion protein.
Immunogen【免疫原】
人p62c-Myc蛋白的合成肽。
Application【应用】
Monoclonal Anti-c-Myc−FITC antibody produced in mouse has been used in immunofluorescence microscopy and immunocytochemistry.
Biochem/physiol Actions【生化/生理作用】
c-Myc plays a vital role in regulation of cell growth, proliferation, differentiation and apoptosis. It is implicated in DNA replication, RNA splicing or transcription. Deregulation in the gene is associated with the development of various types of human cancers. An epitope located within amino acids 410-419, containing the sequence EQKLISEEDL of human c-Myc has been widely used as a tag in many expression vectors, enabling the expression of proteins as c-Myc tag fusion proteins. Epitope tags provide a method to localize gene products in a variety of cell types, to study the topology of proteins and protein complexes, and to identify associated proteins. In addition, it allows characterization of newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available.
Physical form【外形】
0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。
产品性质
Quality Level【质量水平】
200
biological source【生物来源】
mouse
conjugate【偶联物】
FITC conjugate
antibody form【抗体形式】
purified immunoglobulin
antibody product type
primary antibodies
clone【克隆】
9E10, monoclonal
form【形式】
buffered aqueous solution
species reactivity
human
storage condition【储存条件】
protect from light
enhanced validation【增强验证】
recombinant expression
Learn more about Antibody Enhanced Validation
concentration【浓度】
5 μg/mL (suitable for immunoflourescence)
technique(s)
immunocytochemistry: 5.0 μg/mL using mammalian cells transfected with c-Myc tagged fusion protein, fixed with paraformaldehyde.
UniProt accession no.【UniProt登记号】
P01106
shipped in【运输】
dry ice
storage temp.【储存温度】
−20℃
Gene Information
human ... MYC(4609)
产品说明
Storage and Stability【储存及稳定性】
For continuous use, store at 2-8 ℃ for up to one month.For extended storage, freeze in working aliquots.Repeated freezing and thawing is not recommended.Storage in “frost-free” freezers is not recommended. Ifslight turbidity occurs upon prolonged storage, clarifythe solution by centrifugation before use. Workingdilution samples should be discarded if not used within12 hours.
Disclaimer【免责声明】
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
安全信息
Storage Class Code【储存分类代码】
12 - Non Combustible Liquids
WGK
WGK 3
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