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Monoclonal Anti-c-erbB-2 antibody produced in mouse
产品编号: | 3141888 |
规格: | ~2 mg/mL, clone HER2-96, purified immunoglobulin, buffered aqueous solution |
包装规格: | 0.2 ML |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
产品别名
Monoclonal Anti-c-erbB-2 antibody produced in mouse
Anti-HER-2
基本信息
MDL number | MFCD00239647 |
NACRES | NA.41 |
General description【一般描述】 | Monoclonal Anti-c-erbB-2 (HER-2, Neu) (mouse IgG1 isotype) is derived from the (human epidermal growth factor receptor 2) HER2-96 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a purified extracellular domain of human erbB-2, from transfected cells. The human erbB-2 oncogen (also known as HER2, HER-2 or neu) encodes a transmembrane growth factor orphan receptor with a M.W. of 185 kDa (p185). The complete protein consists of an internal cytoplasmic structure with tyrosine kinase activity, a short hydrophobic transmembrane section and an extracellular ligand-binding domain (ECD). |
Immunogen【免疫原】 | purified extracellular domain of human erbB-2 from transfected cells. |
Application【应用】 | Monoclonal Anti-c-erbB-2 antibody produced in mouse has been used in :
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Biochem/physiol Actions【生化/生理作用】 | Erb-b2 receptor tyrosine kinase 2 (ErbB-2) has a pivotal role in signal transduction, where it can stimulate the signal even in the absence of ligand by forming heterodimers with erbB-1/EGF-R, erbB-3, and erbB-4 and hence can modifies ligand affinity. Thus, erbB-2 alters the intracellular responses elicited by EGF and NDF. This control is due to the fact that erbB-2, when in complex with another erbB family receptor, decelerates the rate of ligand dissociation. Therefore, erbB-2 may act as a signalling subunit for other receptors rather than a true growth factor receptor. Because overexpression of the erbB-2 proto-oncogene is frequently associated with an aggressive clinical course of certain human adenocarcinomas, the encoded protein is an attractive target for immunotherapy. Indeed, antibodies to erbB-2 were found to block the crosstalk with growth factor receptors. In addition, receptor overexpression is considered a predictor of poor survival and short time of relapse. |
Physical form【外形】 | 0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。 |
产品性质
Quality Level【质量水平】 | 200 |
biological source【生物来源】 | mouse |
conjugate【偶联物】 | unconjugated |
antibody form【抗体形式】 | purified immunoglobulin |
antibody product type | primary antibodies |
clone【克隆】 | HER2-96, monoclonal |
form【形式】 | buffered aqueous solution |
mol wt【分子量】 | antigen 185 kDa (complete protein) antigen 97-115 kDa (extracellular ligand-binding domain) |
species reactivity | human |
concentration【浓度】 | ~2 mg/mL |
technique(s) | immunoprecipitation (IP): 0.5-1 μg/test using RIPA lysate of cultured CB2 cells (CHO cell line transfected with erbB-2) indirect ELISA: suitable microarray: suitable |
isotype【同位素/亚型】 | IgG1 |
UniProt accession no.【UniProt登记号】 | P04626 |
shipped in【运输】 | dry ice |
storage temp.【储存温度】 | −20℃ |
Gene Information | human ... ERBB2(2064) |
产品说明
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 2 |