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Monoclonal Anti-c-erbB-2 antibody produced in mouse

产品编号:3141888
规格:~2 mg/mL, clone HER2-96, purified immunoglobulin, buffered aqueous solution
包装规格:0.2 ML
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
31418880.2 ML4730
产品别名

Monoclonal Anti-c-erbB-2 antibody produced in mouse

Anti-HER-2

基本信息
MDL number
MFCD00239647
NACRES
NA.41
General description【一般描述】
Monoclonal Anti-c-erbB-2 (HER-2, Neu) (mouse IgG1 isotype) is derived from the (human epidermal growth factor receptor 2) HER2-96 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a purified extracellular domain of human erbB-2, from transfected cells. The human erbB-2 oncogen (also known as HER2, HER-2 or neu) encodes a transmembrane growth factor orphan receptor with a M.W. of 185 kDa (p185). The complete protein consists of an internal cytoplasmic structure with tyrosine kinase activity, a short hydrophobic transmembrane section and an extracellular ligand-binding domain (ECD).
Immunogen【免疫原】
purified extracellular domain of human erbB-2 from transfected cells.
Application【应用】
Monoclonal Anti-c-erbB-2 antibody produced in mouse has been used in :
  • enzyme linked immunosorbent assay (ELISA)
  • immunoprecipitation
  • immunohistochemistry
  • studies on receptor internalization, phosphorylation, ligand binding and tumor growth inhibition

Biochem/physiol Actions【生化/生理作用】
Erb-b2 receptor tyrosine kinase 2 (ErbB-2) has a pivotal role in signal transduction, where it can stimulate the signal even in the absence of ligand by forming heterodimers with erbB-1/EGF-R, erbB-3, and erbB-4 and hence can modifies ligand affinity. Thus, erbB-2 alters the intracellular responses elicited by EGF and NDF. This control is due to the fact that erbB-2, when in complex with another erbB family receptor, decelerates the rate of ligand dissociation. Therefore, erbB-2 may act as a signalling subunit for other receptors rather than a true growth factor receptor. Because overexpression of the erbB-2 proto-oncogene is frequently associated with an aggressive clinical course of certain human adenocarcinomas, the encoded protein is an attractive target for immunotherapy. Indeed, antibodies to erbB-2 were found to block the crosstalk with growth factor receptors. In addition, receptor overexpression is considered a predictor of poor survival and short time of relapse.
Physical form【外形】
0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。
产品性质
Quality Level【质量水平】
200
biological source【生物来源】
mouse
conjugate【偶联物】
unconjugated
antibody form【抗体形式】
purified immunoglobulin
antibody product type
primary antibodies
clone【克隆】
HER2-96, monoclonal
form【形式】
buffered aqueous solution
mol wt【分子量】
antigen 185 kDa (complete protein)
antigen 97-115 kDa (extracellular ligand-binding domain)
species reactivity
human
concentration【浓度】
~2 mg/mL
technique(s)
immunoprecipitation (IP): 0.5-1 μg/test using RIPA lysate of cultured CB2 cells (CHO cell line transfected with erbB-2)
indirect ELISA: suitable
microarray: suitable
isotype【同位素/亚型】
IgG1
UniProt accession no.【UniProt登记号】
P04626
shipped in【运输】
dry ice
storage temp.【储存温度】
−20℃
Gene Information
human ... ERBB2(2064)
产品说明
Disclaimer【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
安全信息
Storage Class Code【储存分类代码】
12 - Non Combustible Liquids
WGK
WGK 2
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