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Monoclonal Anti-Brg1 antibody produced in rat
产品编号: | 3141821 |
规格: | clone 3G4, purified from hybridoma cell culture |
包装规格: | 100 μL |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
产品别名
Monoclonal Anti-Brg1 antibody produced in rat
Anti-BRG1
Anti-RTPS2
Anti-member 4
Anti-protein brahma homolog 1
Anti-transcription activator BRG1
Anti-SNF2
Anti-SMARCA4
Anti-SWI/SNF related
Anti-ATP-dependent helicase SMARCA4
Anti-brahma protein-like 1
Anti-hSNF2b
Anti-SWI2
Anti-sucrose nonfermenting-like 4
Anti-nuclear protein GRB1
Anti-homeotic gene regulator
Anti-BRM/SWI2-related gene 1
Anti-SNF2L4
Anti-actin dependent regulator of chromatin
Anti-BAF190
Anti-SNF2-like 4
Anti-BAF190A
Anti-protein BRG-1
Anti-mitotic growth and transcription activator
Anti-MRD16
Anti-SNF2LB
Anti-subfamily a
Anti-SNF2-BETA
Anti-global transcription activator homologous sequence
Anti-matrix associated
Anti-BRG1-associated factor 190A
基本信息
NACRES | NA.41 |
General description【一般描述】 | Monoclonal Anti-Brg1 (rat IgG1 isotype) is derived from the hybridoma 3G4 produced by the fusion of mouse myeloma cells (SP2) and lymph node cells from rat immunized with a synthetic peptide corresponding to a sequence at the C-terminal region of human Brm-related gene-1 (Brg1) protein. Brg1 or SWI/SNF (SWItch/Sucrose non-fermentable) related, matrix-associated, actin-dependent, regulator of chromatin, subfamily A, member 4 (SMARCA4) gene with 35 exons, is encoded by the gene mapped to human chromosome 19p13. |
Immunogen【免疫原】 | a synthetic peptide corresponding to a sequence at the C-terminal region of human Brg1 protein |
Application【应用】 | Monoclonal Anti-Brg1 antibody produced in rat has been used in:
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Biochem/physiol Actions【生化/生理作用】 | Brm-related gene-1 (Brg1) (or hBrm) protein is the central catalytic ATPase of the SWI/SNF chromatin-remodeling complex, which is involved in transcriptional modulation of hormone-responsive promoters through binding of the complex to various nuclear receptors and its recruitment to gene-specific promoters. In addition, BRG1 has also been shown to act as a transcriptional repressor. It interacts with retinoblastoma tumor suppressor to form a repressor complex which inhibits cell cycle proteins such as cyclins A, D1 and E. BRG1 participates in the firing of origins and the process of replication elongation. This function of BRG1 also facilitates embryogenesis. In addition, It functions as a tumor suppressor and maintains genome stability and prevent cancer. |
Physical form【外形】 | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
产品性质
Quality Level【质量水平】 | 200 |
biological source【生物来源】 | mouse |
antibody form【抗体形式】 | purified immunoglobulin |
antibody product type | primary antibodies |
clone【克隆】 | 3G4, monoclonal |
form【形式】 | buffered aqueous solution |
mol wt【分子量】 | ~185 kDa |
species reactivity | mouse, human |
concentration【浓度】 | ~1 mg/mL |
technique(s) | flow cytometry: 2.5-5 μg/test using HeLa cells immunoblotting: 0.1-0.2.0 μg/mL using HeLa nuclear extract immunofluorescence: 10 μg/mL using HeLa cells |
isotype【同位素/亚型】 | IgG1 |
shipped in【运输】 | dry ice |
storage temp.【储存温度】 | −20℃ |
Gene Information | human ... BRG1(6597) mouse ... Brg1(20586) |
产品说明
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 1 |