Monoclonal Anti-β-Galactosidase-Biotin Conjugate antibody produced in mouse

NA.46

Monoclonal Anti-b-Galactosidase (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. ß-Galactosidase enzyme is encoded by lacZ gene of E. coli.

The antibody may be used for amplification in immunoenzymatic staining by preparing a β-galactosidase anti-β-galactosidase (BGABG) soluble complex. It is not recommended for immunoblotting; it does not recognize denatured or reduced β-galactosidase.

β-galactosidase from E. coli

Monoclonal Anti-β-Galactosidase-Biotin Conjugate antibody produced in mouse may be used with ExtrAvidin-Peroxidase in the dot blot technique on native, purified, or crude β-galactosidase. The antibody was used to detect β-galactosidase by immunofluorescence in paraffin-embedded adult mouse kidney sections. It was used in ELISPOT assays at a dilution of 1:1000.

β-galactosidase enzyme is encoded by lacZ gene of E. coli; it acts on lactose and cleaves it to glucose and galactose. Monoclonal Anti-β-Galactosidase-Biotin Conjugate antibody binds with soluble enzyme even when bound to a surface without causing any loss of enzymatic activity. It is used as readout for promoter activity in lacZ transfected cells.

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.

Prepared by conjugation with ε-aminocaproyl biotin.

200

mouse

biotin conjugate

purified immunoglobulin

primary antibodies

GAL-13, monoclonal

buffered aqueous solution

bacteria

dot blot: 1:2,000

IgG1

dry ice

−20℃

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