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产品性质
biological source【生物来源】 | human breast |
growth mode【生长模式】 | Adherent |
karyotype【核型】 | Range 52-69 |
morphology【形态学】 | Epithelial |
products【产品】 | Not specified |
receptors【受体】 | Not specified |
technique(s) | cell culture | mammalian: suitable |
relevant disease(s)【相关疾病】 | cancer |
shipped in【运输】 | dry ice |
storage temp.【储存温度】 | −196℃ |
基本信息
Cell Line Origin【细胞系来源】 | Human breast medulla carcinoma |
Cell Line Description【细胞系描述】 | Isolated from pleural effusions of a breast cancer patient. Desmosomes, microvilli and tonofilaments observed at boundaries between cells. Ethnicity: Black. |
DNA Profile【DNA图谱分析】 | STR-PCR Data: Amelogenin: X CSF1PO: 10 D13S317: 11 D16S539: 11 D5S818: 12 D7S820: 10,11 THO1: 7,8 TPOX: 9,11 vWA: 15 |
Culture Medium【培养基】 | L15 + 2mM Glutamine + 15% Foetal Bovine Serum (FBS). |
Subculture Routine【传代培养常规】 | Split sub-confluent cultures (70-80%) 1:3 to 1:6, i.e. seeding at 3-6x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 37℃. CO2 is not required. |
Other Notes【其他说明】 | Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information. |
产品说明
Disclaimer【免责声明】 | This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form. |