MCF10A Cells B-RAF V600E

NA.81

MCF10A CELLS B-RAF V600E are pre-neoplastic mammary epithelial cells from a human adult female with a ZFN knock in modification.

Cell freezing medium-DMSO 1X, Cat. No. C6164
Media renewal changes two to three times per week.
The base medium for this cell line is Dulbecco′s Modified Eagle′s Medium/Ham′s Nutrient Mixture F-12, Cat. No. 51448C. To make the complete growth medium, add the following components to the base medium: horse serum, Cat No. H1270, to a final concentration (v/v) of 5%; cholera toxin, Cat No. C8052, to a final concentration of 1 ng/mL; human insulin, Cat No. I9278, to a final concentration of 10 ug/mL; epidermal growth factor, Cat No. E9644, to a final concentration of 10 ng/mL; and hydrocortisone, Cat No. H6909, to a final concentration of 0.5 ug/mL.
Rapidly thaw vial by gentle agitation in 37℃ water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°

Zinc finger nuclease (ZFN) knockin on chromosome 7q34
These MCF10A cells are adherent with a doubling time of approx. 20 hours

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, cytochrome oxidase I (COI) analysis for cell line species confirmation.

组份可单独销售
MCF10A CONTROL CELLS 1 vial

200

human male mammary glands (Source Disease: Fibrocystic Disease)

164757

−196℃

human ... BRAF(673)

10 - Combustible liquids

WGK 3