LuminoCt^® qPCR ReadyMix^TM

NA.55

LuminoCt^® qPCR ReadyMix combines the performance enhancements of our JumpStart^™ Taq antibody for hot start PCR with the convenience of an easy-to-use reaction mixture. This is the ideal solution for performing high-throughput, quantitative PCR methods that rely on a fluorescent probe.

LuminoCt^® qPCR ReadyMix^™ has been used in 2-step quantitative reverse transcription polymerase chain reaction (qRT-PCR) assay to quantify the reverse transcription product or cDNA for gene expression analysis.
LuminoCt qPCR ReadyMix is designed to deliver unsurpassed assay speeds without sacrificing accuracy, precision, or sensitivity. Extensive design and validation of the ReadyMix chemistry has virtually eliminated the need for optimization when LuminoCt is used in conjunction with properly designed primers and probes. Inclusion of JumpStart^™ Taq antibody in the ReadyMix eliminates polymerase activity at ambient temperatures without causing the performance issues associated with chemically modified hot-start Taq. This allows for very rapid activation of the Taq and delivers unparalleled assay sensitivity, while allowing for benchtop reaction setup.

• Kit is designed to perform probe-based qPCR assays on commonly available real-time instrument platforms.
• ReadyMix requires the addition of reference dye (provided in kit) when being used on real-time instruments that require ROX passive reference dye for normalization of qPCR assays.
• Kit is not compatible with qPCR instruments that utilize glass capillary tubes.

• Commercially available 5′ nuclease assays (e.g., TaqMan^® Assays) are compatible with LuminoCt ReadyMix.
• At a minimum, use software to design primers and probes for assays and ensure that qPCR amplicons are <200 bp.
• Sigma recommends designing primers and probes with Beacon Designer software

To order primers and probes from Sigma, visit www.sigma.com/oligos

Eppendorf is a registered trademark of Eppendorf AG
JumpStart is a trademark of sigma-aldrich Co. LLC
LuminoCt is a registered trademark of sigma-aldrich Co. LLC
ReadyMix is a trademark of sigma-aldrich Co. LLC
TaqMan is a registered trademark of Roche Molecular Systems, Inc.

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R4526 100X ROX internal reference dye

200

liquid

sufficient for 100 reactions
sufficient for 2000 reactions
sufficient for 500 reactions

dNTPs included
hotstart

qPCR: suitable

colorless

purified DNA

agriculture

for use with ABI 5700
for use with ABI 7000
for use with ABI 7300
for use with ABI 7500 Fast
for use with ABI 7500
for use with ABI 7700
for use with ABI 7900 Fast
for use with ABI 7900 HT
for use with ABI 7900
for use with ABI StepOne
for use with ABI StepOnePlus
for use with ABI ViiA 7
for use with Bio-Rad CFX384
for use with Bio-Rad CFX96
for use with Bio-Rad MJ Chromo4
for use with Bio-Rad MJ Opticon 2
for use with Bio-Rad MJ Opticon Cepheid SmartCycler
for use with Bio-Rad MJ Opticon
for use with Bio-Rad MiniOpticon
for use with Eppendorf^® Mastercycler ep realplex2 s
for use with Eppendorf^® Mastercycler ep realplex
for use with Illumina Eco qPCR
for use with Qiagen Corbett Rotor-Gene 3000
for use with Qiagen Corbett Rotor-Gene 6000
for use with Qiagen Corbett Rotor-Gene Q
for use with Roche LightCycler 480
for use with Strategene Mx3000P
for use with Strategene Mx3005P
for use with Strategene Mx4000

probe-based

wet ice

−20℃

LuminoCt qPCR ReadyMix, Catalog Number L5794, contains optimized concentrations of Tris-HCl, pH 8.3, KCl, dNTPs (dATP, dCTP, dGTP, TTP), stabilizers, MgCl₂ and JumpStart Taq DNA Polymerase. Provided as 100, 500 and 2000 reactions (25 μL mix in a 50 μL reaction volume).

100x ROX internal reference dye, Catalog Number R4526. Optional, for use with machines compatible with an internal reference dye, e.g., ABI and Stratagene.

Default reaction volume is 50 μL

100RXN is packaged as 1 X 2.5 mL
500RXN is packaged as 1 X 12.5 mL
2000RXN is packaged as 1 X 50 mL

10 - Combustible liquids

WGK 1