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Lambda DNA Mixed Digest
基本信息
NACRES | NA.25 |
General description【一般描述】 | Sigma′s Lambda DNA Mixed Digest contains 10 fragments from 1,503 − 48,502 bp. Six ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel. |
Application【应用】 | Lambda DNA Mixed Digest has been used for DNA electrophoresis. Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels. |
Components【组分】 | 10mM Tris-HCl 中的溶液,pH 8.0,1mM EDTA |
Preparation Note【制备说明】 | Bacteriophage lambda DNA was completely digested with by Apa I, Kpn I, Xba I and Xho I in separate reactions, sticky ends filled with DNA polymerase I Klenow (to prevent reannealing), mixed with uncut bacteriophage lambda DNA, and then purified by phenol extraction. |
Other Notes【其他说明】 | For optimal resolution, the recommended agarose gel concentration is 0.4%. |
产品性质
Quality Level【质量水平】 | 200 100 |
form【形式】 | buffered aqueous solution |
usage【用途】 | 100 uses (0.1 mg size) 20 uses (20 μg size) |
suitability【适用性】 | suitable for electrophoresis (DNA) |
storage temp.【储存温度】 | −20℃ |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |