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Lambda DNA Mixed Digest

产品编号:3104280
规格:for DNA electrophoresis
包装规格:20 μG,0.1 MG
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
310428020 μG948
31042800.1 MG3570
基本信息
NACRES
NA.25
General description【一般描述】
Sigma′s Lambda DNA Mixed Digest contains 10 fragments from 1,503 − 48,502 bp. Six ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.
Application【应用】
Lambda DNA Mixed Digest has been used for DNA electrophoresis.
Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.
Components【组分】
10mM Tris-HCl 中的溶液,pH 8.0,1mM EDTA
Preparation Note【制备说明】
Bacteriophage lambda DNA was completely digested with by Apa I, Kpn I, Xba I and Xho I in separate reactions, sticky ends filled with DNA polymerase I Klenow (to prevent reannealing), mixed with uncut bacteriophage lambda DNA, and then purified by phenol extraction.
Other Notes【其他说明】
For optimal resolution, the recommended agarose gel concentration is 0.4%.
产品性质
Quality Level【质量水平】
200
100
form【形式】
buffered aqueous solution
usage【用途】
100 uses (0.1 mg size)
20 uses (20 μg size)
suitability【适用性】
suitable for electrophoresis (DNA)
storage temp.【储存温度】
−20℃
安全信息
Storage Class Code【储存分类代码】
12 - Non Combustible Liquids
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