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乳酸脱氢酶,重组

Lactic Dehydrogenase, recombinant from E. coli
产品编号:3104245
规格:≥90 U/mg
CAS NO:9001-60-9
包装规格:25 MG,100 MG
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品编号包装单位单价(元)国内现货国外库存询价单
310424525 MG3200
3104245100 MG10010
产品别名

9001-60-9

乳酸脱氢酶,重组

Lactic Dehydrogenase, recombinant from E. coli

(S)-Lactate: NAD+ oxidoreductase

Lactate

L-Lactate Dehydrogenase

( S )-乳酸盐:NAD + 氧化还原酶

乳酸

L- 乳酸脱氢酶

基本信息
Enzyme Commission (EC) Number【酶学委员会(EC)编号】
1.1.1.27 ( BRENDA | IUBMB )
EC number【EC 号】
232-617-8
MDL number
MFCD00131466
NACRES
NA.54
General description【一般描述】
LDH (lactic dehydrogenase), a glycolytic enzyme, particularly present in skeletal muscle, heart, liver, kidneys, brain, lungs and red blood cells. It has five isoenzyme forms. LDH possess a tetrameric structure.
Application【应用】
Lactic Dehydrogenase, recombinant from E. coli has been used:
  • in lactate dehydrogenase (LDH) and malate dehydrogenase 1 (MDH1)assays and cross-linking assays
  • to prepare assay buffer to measure pyruvate kinase (PYK) by coupled assay
  • in in vitro DltC D-alanylation assay

Biochem/physiol Actions【生化/生理作用】
Conversion of L-lactate into L-pyruvate is crucial in hypoxic and anaerobic conditions, especially when synthesis of adenosine triphosphate (ATP) by oxidative phosphorylation is interrupted.
L -乳酸脱氢酶催化 L -乳酸转化为 L -丙酮酸,同时还原 NAD + 为 NADH 和 H +
Unit Definition【单位定义】
One unit corresponds to the amount of enzyme which reduces 1 μmol pyruvate per minute at pH 7.4 and 25℃ (NADH as cofactor)
产品性质
Quality Level【质量水平】
100
recombinant【重组】
expressed in E. coli
form【形式】
powder
Specific Activity
≥90 U/mg
storage temp.【储存温度】
−20℃
安全信息
Storage Class Code【储存分类代码】
13 - Non Combustible Solids
WGK
WGK 3
Personal Protective Equipment【个人防护装备】
Eyeshields, Gloves, type N95 (US)
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