您的位置:首页 > 产品中心 > L6.G8.C5
产品性质
biological source【生物来源】 | rat muscle |
growth mode【生长模式】 | Adherent |
karyotype【核型】 | Not specified |
morphology【形态学】 | Fibroblast |
products【产品】 | Not specified |
receptors【受体】 | Not specified |
technique(s) | cell culture | mammalian: suitable |
shipped in【运输】 | dry ice |
storage temp.【储存温度】 | −196℃ |
基本信息
Cell Line Origin【细胞系来源】 | Rat skeletal muscle myoblast |
Cell Line Description【细胞系描述】 | A subclone of L6, a rat thigh muscle cell line. On reaching confluence the cells will fuse to form myotubes and twitching movement will be seen. Once cultures have fused they cannot be recloned. |
Culture Medium【培养基】 | DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS). |
Subculture Routine【传代培养常规】 | Split semi-confluent cultures to a seeding density of 1.5-2x1,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37℃. Cells fuse on reaching confluence, producing myotubes. To prevent fusion maintain the cultures sub-confluent and reclone every 6-8 weeks. |
Other Notes【其他说明】 | Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information. |